FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631416
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACGATTCT22170.35111558782165797No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21800.3452557426482699No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC21380.3386040265055051No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18290.28966640059802096No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC17150.27161174249623066No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16270.25767481343519966No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG16150.25577432310869536No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15150.23993690372116008No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14870.23550242629265017No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCCGCTAGG13740.21760614238473525No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12290.19464188427280904No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA12290.19464188427280904No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12250.19400838749730764No Hit
ATTGACAACAGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11560.18308056811990825No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACGATTCTTTGACATACT10790.1708857551915061No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTTT10780.1707273809976307No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10370.16423403904874123No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9610.15219760031421442No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG9420.1491884906305827No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9240.14633775514082634No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9170.14522913578369887No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTAAAGG9130.14459563900819744No Hit
ATTTACAGGTAGTTTCCGAAAACATTGCTTGAGTTTTAGAACAATATGTT8650.1369936777021805No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8650.1369936777021805No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8480.1343013164062995No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG8470.13414294221242415No Hit
GGTTAGGATAAAACCTAGCGGGCATAATTAATTTTAATCTCCTTTTTTCA8460.1339845680185488No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8420.13335107124304738No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8210.13002521317166496No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8090.12812472284516072No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8010.1268577292941579No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7790.12337349702890013No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7780.12321512283502477No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7720.12226487767177266No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7670.12147300670239589No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7580.1200476389575177No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7520.1190973937942656No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7450.11798877443713811No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC7410.1173552776616367No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7300.11561316152900782No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7190.11387104539637893No Hit
GGGTAAAGGTGCAGATATTGATTACGCAGAAAGGCCTTGTAAGTTGGCGA7190.11387104539637893No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7090.11228730345762541No Hit
ATCAATATCTGCACCTTTACCCCGTTGCCAGGCAACGGTCCGGTCTCTGC7080.11212892926375005No Hit
TCCTCAGGCCATGCAGTGGAATTCCACCACATTCCATCAAGCTCTGCTAG7040.11149543248824864No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGTAGTTTCCGAAAAC7010.11102030990662258No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6960.11022843893724582No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA6930.10975331635561976No Hit
TGATAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCT6750.10690258086586339No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6710.10626908409036198No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6540.10357672279448096No Hit
GTCTATTTACAGGTAGTTTCCGAAAACATTGCTTGAGTTTTAGAACAATA6490.10278485182510422No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6470.1024681034373535No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATGTGG6430.10183460666185208No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6370.10088436149859997No Hit
CCTTTACCCCGTTGCCAGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG6370.10088436149859997No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6320.1000924905292232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)