FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631416
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG25120.39783597501488716No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACGATTCT20690.32767620712810575No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC20050.3175402587200831No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT18540.29362575544490477No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG17500.277154839281868No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16980.2689193812003497No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC15350.24310438759866712No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15020.23787803920078046No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13530.21428028431335283No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCCGCTAGG13040.20651994881346053No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACGATTCTTTGACATACT12470.19749261976256544No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12030.19052415523204985No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA11470.1816552003750301No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTTT10970.17373649068126243No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10890.1724694971302596No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10750.17025225841600466No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT10460.16565940679361943No Hit
ATTGACAACAGTGCCAGCGGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10380.16439241324261658No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG10270.1626502971099877No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9540.15108898095708692No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTAAAGG9430.14934686482445803No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9130.14459563900819744No Hit
ATTTACAGGTAGTTTCCGAAAACATTGCTTGAGTTTTAGAACAATATGTT9060.14348701965107No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8780.13905254222256008No Hit
GGTTAGGATAAAACCTAGCGGGCATAATTAATTTTAATCTCCTTTTTTCA8660.13715205189605587No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8550.13540993576342697No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8340.13208407769204455No Hit
GTCTATTTACAGGTAGTTTCCGAAAACATTGCTTGAGTTTTAGAACAATA8320.13176732930429383No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8240.130500335753291No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8150.12907496800841284No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG7990.1265409809064072No Hit
TTCTTCTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7920.1254323615492797No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7900.125115613161529No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7550.11957251637589165No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7450.11798877443713811No Hit
ATCAATATCTGCACCTTTACCCCGTTGCCAGGCAACGGTCCGGTCTCTGC7420.11751365185551205No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA7400.11719690346776136No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7380.11688015508001064No Hit
CTACTGTACCTATCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7330.11608828411063386No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7250.11482129055963104No Hit
TCCTCAGGCCATGCAGTGGAATTCCACCACATTCCATCAAGCTCTGCTAG7210.11418779378412963No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7190.11387104539637893No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7170.11355429700862822No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7130.1129208002331268No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7100.11244567765150075No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGTAGTTTCCGAAAAC7070.11197055506987469No Hit
CCTTTACCCCGTTGCCAGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG6860.10864469699849227No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6840.10832794861074158No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6810.10785282602911551No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATGTGG6740.10674420667198803No Hit
GGGTAAAGGTGCAGATATTGATTACGCAGAAAGGCCTTGTAAGTTGGCGA6660.10547721312098521No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6450.10215135504960281No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6410.1015178582741014No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG6400.10135948408022603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)