FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450614
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG28540.6333580403627052No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT23360.5184037779562997No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA18260.40522487095385407No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG16120.3577341138979259No Hit
ATCCTAACATTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15410.34197783468778153No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC13710.30425153235363306No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC13680.30358577407714804No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC12860.2853883811865588No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12750.2829472675061139No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12550.27850887899621407No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAATGTT12360.2742924099118092No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG12070.26785674657245445No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11970.2656375523175046No Hit
TGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA11800.2618649220840897No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC11470.254541581042755No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10890.2416702543640455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10860.24100449608756053No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10650.23634418815216574No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10250.22746741113236607No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9830.21814679526157643No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA9820.21792487583608142No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG9770.21681527870860648No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA9540.21171113192222168No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT9470.21015769594375674No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATGTCCTCCTGT9060.2010589994984621No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA8950.19861788581801718No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA8770.19462333615910737No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA8690.19284798075514742No Hit
GTTGTCAACATGCCCTGAGCCTGAGGGCTCCACCCTAAAAGACCGCCGTG8640.19173838362767245No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8600.1908507059256925No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8220.18241776775688281No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7990.17731362097049805No Hit
CCCTAAAAGACCGCCGTGTGGAGGGGTGAACCCTGGCCCGAATGCTCCCG7910.1755382655665381No Hit
GTTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7850.17420674901356814No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7750.17198755475861824No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT7540.16732724682322342No Hit
GATTAATTATGCCTGCTAGGTTCTATCCTAACATTACCAAATATTTGCCC7520.16688340797223344No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC7440.16510805256827352No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7370.16355461658980858No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7330.1626669388878286No Hit
GTTCTATCCTAACATTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7310.16222310003683862No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT7030.15600935612297887No Hit
ATATTAACGTTGACATAGCCGACTACTGCTTCCCTGGATGCTGGGTCTTC6990.15512167842099892No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6860.15223672588956402No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA6810.15112712876208906No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC6700.14868601508164417No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGGGAGTCCAA6620.14691065967768424No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA6590.14624490140119925No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG6580.14602298197570426No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCCCTTAGAGGTGG6580.14602298197570426No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC6550.14535722369921927No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6460.14335994886976436No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6460.14335994886976436No Hit
CTTTTAGGGTGGAGCCCTCAGGCTCAGGGCATGTTGACAACAGTGCCAGC6450.14313802944426937No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCGCACTCAGGCA6430.1426941905932794No Hit
CTCCACAACAGTCCACCAAGCTCCTGCTGGTGGCTCCAGTTCCGGAACAG6390.14180651289129942No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA6360.14114075461481446No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT6270.13914347978535951No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6220.13803388265788458No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTGAGA6130.13603660782842966No Hit
CTTTACCACAGGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAA6040.13403933299897475No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6040.13403933299897475No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC6010.13337357472248976No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5990.13292973587149978No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA5910.13115438046753986No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5810.12893518621258993No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG5780.12826942793610496No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5730.12715983080863No Hit
AATTATGCCTGCTAGGTTCTATCCTAACATTACCAAATATTTGCCCTTGG5710.12671599195764002No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5620.1247187171281851No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5570.12360912000071016No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5560.12338720057521516No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5540.12294336172422517No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACAGTCCACCAAGCTCCTGCTG5530.12272144229873018No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5520.12249952287323518No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCGATGTCAACGACCGACCT5420.12028032861828528No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5400.11983648976729529No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5390.11961457034180029No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAAT5370.11917073149081032No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5310.11783921493784036No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAATA5300.11761729551234537No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5240.11628577895937543No Hit
ACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACA5220.11584194010838544No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCCTACAGACCACCAAATGCC5200.11539810125739546No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5200.11539810125739546No Hit
GGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC5180.1149542624064055No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5180.1149542624064055No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5150.1142885041299205No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT5130.11384466527893053No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC5070.11251314872596059No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGGTT5070.11251314872596059No Hit
CATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5040.1118473904494756No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5010.11118163217299062No Hit
GCTCTGTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4910.10896243791804071No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGT4850.10763092136507077No Hit
GATTAAGATTAATTATGCCTGCTAGGTTCTATCCTAACATTACCAAATAT4790.10629940481210083No Hit
ATTTACAGGAGGACATTATCAATAGATGTCAACAATATGTGGGCCCTCTT4760.10563364653561585No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4730.10496788825913086No Hit
CCTTTATCCAAGGGCAAATATTTGGTAATGTTAGGATAGAACCTAGCAGG4720.10474596883363588No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTG4590.101861016302201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)