FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450614
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG30540.6777419254617034No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT22920.50863932323452No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA16820.3732684736825753No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG16000.355071080791986No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT15470.3433093512407515No Hit
ATCCTAACATTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT14270.3166790201813526No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC14210.3153475036283826No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC14190.3149036647773926No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12980.2880514142924987No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12440.27606776531576915No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12320.27340473220982925No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG12060.26763482714695946No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAATGTT11910.2643060357645346No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11400.25298814506429007No Hit
TGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA10900.2418921737895405No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10860.24100449608756053No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA10710.23767570470513566No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10650.23634418815216574No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG10310.228798927685336No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10240.22724549170687106No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC10060.22325094204796123No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA9660.21437416502816156No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTT9550.21193305134771667No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9380.20816042111430183No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATGTCCTCCTGT9370.20793850168880681No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA8680.19262606132965243No Hit
ATATTAACGTTGACATAGCCGACTACTGCTTCCCTGGATGCTGGGTCTTC8410.18663423684128766No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT8300.18419312316084274No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA7990.17731362097049805No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC7970.17686978211950805No Hit
CCCTAAAAGACCGCCGTGTGGAGGGGTGAACCCTGGCCCGAATGCTCCCG7940.17620402384302308No Hit
GTTGTCAACATGCCCTGAGCCTGAGGGCTCCACCCTAAAAGACCGCCGTG7930.17598210441752807No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7690.17065603820564829No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA7610.1688806828016884No Hit
GATTAATTATGCCTGCTAGGTTCTATCCTAACATTACCAAATATTTGCCC7480.16599573027025347No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7480.16599573027025347No Hit
GTTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7450.1653299719937685No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7370.16355461658980858No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7330.1626669388878286No Hit
CTCAAGAACAGTGTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACA7250.16089158348386867No Hit
GTTCTATCCTAACATTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7090.15734087267594882No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7060.15667511439946383No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTTTGTC6930.15379016186802896No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT6880.152680564740554No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTGTCTCTTCCAAAAGTGAGA6850.152014806464069No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC6820.15134904818758405No Hit
CTTTTAGGGTGGAGCCCTCAGGCTCAGGGCATGTTGACAACAGTGCCAGC6790.15068328991109908No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6700.14868601508164417No Hit
CTCCACAACAGTCCACCAAGCTCCTGCTGGTGGCTCCAGTTCCGGAACAG6650.1475764179541692No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCGCACTCAGGCA6570.14580106255020928No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6420.1424722711677844No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCGATGTCAACGACCGACCT6280.13936539921085453No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6250.13869964093436957No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGGGAGTCCAA6230.13825580208337956No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6180.1371462049559046No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA6170.1369242855304096No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA6130.13603660782842966No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCCCTTAGAGGTGG6120.13581468840293467No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6100.13537084955194467No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6090.13514893012644968No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAAT6070.1347050912754597No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6070.1347050912754597No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA5890.13071054161654985No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5890.13071054161654985No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5700.12649407253214504No Hit
CTTTACCACAGGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAA5690.12627215310665002No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT5690.12627215310665002No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5660.12560639483016506No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAATA5650.12538447540467007No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5650.12538447540467007No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG5520.12249952287323518No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5470.12138992574576024No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5440.12072416746927525No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG5430.12050224804378026No Hit
GGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC5330.11828305378883035No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5270.11695153723586041No Hit
CCTTTATCCAAGGGCAAATATTTGGTAATGTTAGGATAGAACCTAGCAGG5230.11606385953388043No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTACCGC5200.11539810125739546No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCCTACAGACCACCAAATGCC5200.11539810125739546No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5190.11517618183190047No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5130.11384466527893053No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5110.11340082642794054No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5060.11229122930046559No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5010.11118163217299062No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC4990.11073779332200066No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4980.11051587389650566No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4930.1094062767690307No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTG4900.10874051849254572No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4870.10807476021606076No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT4870.10807476021606076No Hit
CATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4870.10807476021606076No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4830.10718708251408078No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACAGTCCACCAAGCTCCTGCTG4710.10452404940814089No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4680.10385829113165593No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGT4670.10363637170616093No Hit
AATTATGCCTGCTAGGTTCTATCCTAACATTACCAAATATTTGCCCTTGG4640.10297061342967595No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4640.10297061342967595No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4570.10141717745121101No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC4520.10030758032373605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)