FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454026
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG15600.3435926576892072No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG14560.32068648050992676No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14270.3142991811041658No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12650.27861840511336355No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11700.2576944932669054No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG9510.20945937016822827No Hit
GTATTCATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAAC9420.2074771048354059No Hit
CTTACAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCT9090.20020879861505728No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8900.19602401624576565No Hit
GTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8780.19338099580200255No Hit
CCTACACATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT8570.18875571002541705No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG8560.18853545832177013No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8380.18457092765612543No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA8310.18302916573059694No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8000.17620136291754215No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7930.17465960099201366No Hit
ACCTATCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATT7880.17355834247377902No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT7700.16959381180813435No Hit
GTATACATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAAC7360.1621052538841388No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC7330.161444498773198No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7160.15770021981120025No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7040.15505719936743712No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT6910.15219392722002706No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6790.14955090677626393No Hit
GGTACGGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTT6670.1469078863325008No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA6580.14492562099967843No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC6560.14448511759238458No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6450.14206234885226837No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAT6410.14118134203768065No Hit
GTATGTAAATAATGCCTAGTTTGGAAATAATGATTAACTATGTGTTCAGG6190.13633580455744826No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5820.1281864915225119No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5760.12686498130063034No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5760.12686498130063034No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCT5400.11893591996934097No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5390.11871566826569403No Hit
GACTTCTTTCCTTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTA5390.11871566826569403No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5360.11805491315475325No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5290.11651315122922475No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5290.11651315122922475No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5200.11453088589640241No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5140.11320937567452084No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA5060.11144736204534542No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5010.11034610352711079No Hit
TGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCAT4990.10990560011981693No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA4930.10858408989793536No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4910.10814358649064151No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4900.10792333478699458No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4890.10770308308334765No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT4860.10704232797240686No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA4830.10638157286146609No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4810.10594106945417223No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4770.10506006263958452No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4740.10439930752864372No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC4710.10373855241770295No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4680.10307779730676216No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4650.10241704219582139No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4650.10241704219582139No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG4630.10197653878852753No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4620.10175628708488062No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4590.10109553197393982No Hit
CTAGTGGACCTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGA4570.10065502856664596No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4550.1002145251593521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)