FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454026
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG15680.3453546713183826No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG14910.3283952901375692No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14130.31121565725310885No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11470.25262870408302607No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT10040.22113271046151542No Hit
GTATTCATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAAC9880.21760868320316457No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9360.2061555946135243No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG8770.1931607440983556No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG8600.18941646513635785No Hit
CTTACAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCT8590.1891962134327109No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8440.185892437878007No Hit
GTATGTAAATAATGCCTAGTTTGGAAATAATGATTAACTATGTGTTCAGG8010.1764216146211891No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7980.1757608595102483No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7960.17532035610295446No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7860.17311783906648517No Hit
CCTACACATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT7640.16827230158625278No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA7500.16518877773519577No Hit
GTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7460.16430777092060808No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT7340.16166475047684495No Hit
GTATACATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAAC7340.16166475047684495No Hit
ACCTATCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATT7340.16166475047684495No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7030.1548369476637902No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7030.1548369476637902No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA6380.14052058692673988No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC6360.14008008351944604No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG6210.1367763079647421No Hit
GGTACGGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTT6010.13237127389180356No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC5850.1288472466334527No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAT5640.12422196085686724No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT5620.12378145744957338No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCT5590.12312070233863259No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5550.12223969552404487No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5520.1215789404131041No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5380.11849541656204711No Hit
GACTTCTTTCCTTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTA5330.11739415804381247No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5330.11739415804381247No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG5310.1169536546365186No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5310.1169536546365186No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5300.11673340293287168No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5270.1160726478219309No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA5270.1160726478219309No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT5140.11320937567452084No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5110.11254862056358007No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5100.11232836885993314No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA5060.11144736204534542No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT4960.10924484500887614No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4900.10792333478699458No Hit
GTATGCAAATAATGCCTAGTTTGGAAATAATGATCAACTATGTGTTCAGG4890.10770308308334765No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4800.10572081775052529No Hit
AATGAATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAT4780.10528031434323143No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4750.10461955923229066No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA4730.10417905582499681No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4730.10417905582499681No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4680.10307779730676216No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4590.10109553197393982No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4580.1008752802702929No Hit
GTCTATGTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATA4580.1008752802702929No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC4560.10043477686299904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)