FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390079
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14380.36864327482381776No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG13190.33813663386134607No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG11150.28583953506853743No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG10220.2619982106188746No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT9880.2532820274867399No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9740.24969301090291968No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9340.23943867780629055No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC9250.23713145285954895No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8610.22072451990494232No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8540.21893001161303222No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA8510.21816093663078506No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC8430.2161100700114592No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA8260.21175197844539184No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7800.19995949538426833No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7480.191756028906965No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT7440.19073059559730207No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG7400.18970516228763917No Hit
CTGTAAGAGGGCCCACATATTGTTGGCATCTGGTGATAATGTCCTCTTTC7360.18867972897797627No Hit
ATCTAAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG7220.18509071239415606No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7190.18432163741190888No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT7110.18227077079258303No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA6680.17124736271370672No Hit
ATATGTTCCTGTGGTAAGGTGCCCCAACTTCCAATTACATATCCCATGAA6540.1676583461298865No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6510.16688927114763932No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTACCACAG6420.16458204620089775No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGATAAAGGCATTAAAC6380.16355661289123485No Hit
CCTTACCACAGGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAA6290.16124938794449328No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTAGTAAGGTT6220.1594548796525832No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA6180.15842944634292028No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6100.15637857972359445No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA6070.15560950474134727No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5910.15150777150269562No Hit
CCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCG5850.14996962153820123No Hit
AGAGATTGGTGAGTGATTGGAGGTTGGGGACTGCAAATTTTGGCCAGGAC5740.14714967993662822No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5730.14689332160921248No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5710.14638060495438102No Hit
CACATATTGTTGGCATCTGGTGATAATGTCCTCTTTCAAATGAATGTGAG5700.1461242466269653No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT5560.1425352300431451No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT5530.1417661550608979No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5470.14022800509640354No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5470.14022800509640354No Hit
CATGTATACAAGCTAGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5410.13868985513190918No Hit
GTGGTAAGGTGCCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5110.1309991053094373No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4970.1274100887256171No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4920.12612829708853848No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4790.122795638832134No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAAT4760.12202656384988683No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4730.12125748886763962No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4690.12023205555797671No Hit
ATGTAAGACCTTGGGCAAGACCTGGCAGACGTTCACGGTGGTTTCCATGC4620.11843754726606662No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACA4580.11741211395640369No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT4520.11587396399190934No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA4500.11536124733707788No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTAGTA4480.11484853068224642No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4420.11331038071775204No Hit
CATTATCACCAGATGCCAACAATATGTGGGCCCTCTTACAGTTAACGAAA4320.11074679744359475No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC4300.11023408078876329No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGTCTAGT4270.10946500580651611No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4160.1066450642049431No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4150.10638870587752738No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4110.10536327256786447No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4100.10510691424044873No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4100.10510691424044873No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4050.1038251226033701No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4030.10331240594853863No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA3950.1012615393292128No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3950.1012615393292128No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA3920.10049246434696561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)