FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467217
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA18560.39724581939441417No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13510.28915899892341246No Hit
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13160.2816678331481945No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG12670.2711802010628894No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTGCGAGAAAGGACAACGGAGT12010.257054002743907No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11630.24892073704509896No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10950.2343664721103898No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10950.2343664721103898No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG10560.22601917310371838No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10370.22195254025431438No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTATATCGGGAGGCCTTAGA10270.2198122071756807No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9460.20247550923874774No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9180.19648257661857338No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9110.1949843434635298No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8670.18556687791754153No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAGCCTTAT8640.18492477799395143No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA8270.17700554560300674No Hit
GCATCAAACCTCGAAAAGGCATGGGGACGAACCTTTCTGTTCCCAATCCT8210.1757213457558265No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8060.17251084613787598No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCA8000.17122664629069576No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7900.16908631321206205No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7680.16437758043906794No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGACGAACCTT7480.16009691428180053No Hit
GTTGTCAATATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG7480.16009691428180053No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA7480.16009691428180053No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7430.15902674774248368No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7340.15710044797171335No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7310.15645834804812325No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7200.15410398166162617No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7180.15367591504589945No Hit
TAGACAAAGGCATTAAGCCTTATTATCCGGAACATGCAGTTAATCATTAC7010.15003734881222217No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6940.14853911565717856No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA6600.141261983189824No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6600.141261983189824No Hit
TACCCTGGAAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6570.14061988326623387No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA6560.14040584995837052No Hit
GCAGACGCAGAAGGGGACGATAGAGTCCCAAGCGGCCGCGAGACGGATCG6540.13997778334264377No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGATCCGTCTCG6500.13912165011119032No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA6390.13676728372469324No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTTAT6310.1350550172617863No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6300.1348409839539229No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG6300.1348409839539229No Hit
TCCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6290.13462695064605953No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6080.13013225118092878No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6040.1292761179494753No Hit
ATATAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT5980.12799191810229507No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5880.12585158502366137No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5880.12585158502366137No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5870.12563755171579802No Hit
CTCTTACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG5870.12563755171579802No Hit
ATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAACGAGAGTAACTCCA5840.1249954517922079No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5840.1249954517922079No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGATCCGTCTCGCGGCCG5790.12392528525289105No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTTCCTGTAA5700.12199898548212072No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5660.12114285225066726No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5630.12050075232707715No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5580.11943058578776028No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA5560.11900251917203356No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5540.11857445255630682No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5530.11836041924844344No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5520.11814638594058006No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5420.11600605286194639No Hit
ACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC5350.11450781970690278No Hit
CCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAAGAGTCCGCTTATGC5310.1136516864754493No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTT5240.11215345332040573No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5160.11044118685749876No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC5140.11001312024177202No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5130.10979908693390865No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA5100.10915698701031853No Hit
CAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG5090.10894295370245519No Hit
GCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCC5020.10744472054741158No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5010.1072306872395482No Hit
GTCCAAGAGTCCGCTTATGCAAGACCTTGGGCAAGACCTGGTGGGCGTTC4990.10680262062382148No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTTCTG4980.1065885873159581No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4980.1065885873159581No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4970.10637455400809474No Hit
ATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4970.10637455400809474No Hit
CTGCATCTGCCGTACCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT4970.10637455400809474No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGTACGG4960.10616052070023137No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4940.10573245408450464No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4930.10551842077664127No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGGA4900.10487632085305115No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4880.10444825423732441No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4830.10337808769800756No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4750.10166582123510061No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4740.10145178792723723No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTTATGGGATATGTAA4700.10059565469578377No Hit
GATCCATACTGCGGAACTCCTGGCGGCTTGTTTTGCTCGCAGCCGGTCTG4700.10059565469578377No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4690.1003816213879204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)