FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467217
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA16180.34630589212293217No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13410.28701866584477875No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG12340.2641171019033982No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG11980.2564119028203169No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11650.2493488036608257No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTGCGAGAAAGGACAACGGAGT11270.24121553796201764No Hit
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10430.22323674010149458No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10150.21724380748132024No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9800.2097526417061023No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9710.20782634193533198No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9310.1992650096207972No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9300.1990509763129338No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8760.18749317768831186No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTATATCGGGAGGCCTTAGA8760.18749317768831186No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8610.1842826780703613No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA8590.18385461145463455No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8230.17614941237155327No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA8060.17251084613787598No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7780.16651791351770162No Hit
CTGCATCTGCCGTACCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT7390.1581706145110302No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCA7270.15560221481666978No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7180.15367591504589945No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAGCCTTAT7180.15367591504589945No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7160.1532478484301727No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7110.15217768189085584No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6940.14853911565717856No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGATCCGTCTCGCGGCCG6750.14447248280777453No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6710.14361634957632108No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6650.14233214972914085No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6600.141261983189824No Hit
GCATCAAACCTCGAAAAGGCATGGGGACGAACCTTTCTGTTCCCAATCCT6600.141261983189824No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTTTTGCCTTCTG6580.14083391657409725No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGATCCGTCTCG6480.13869358349546357No Hit
GTTGTCAATATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG6380.13655325041682986No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6380.13655325041682986No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6340.1356971171853764No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA6310.1350550172617863No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6280.13441291733819616No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA6230.13334275079887933No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6170.1320585509516991No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6150.13163048433597235No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6140.131416451028109No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCGTTT6090.13034628448879212No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG6060.12970418456520202No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6010.1286340180258852No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTTAT5950.12734981817870497No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGACGAACCTT5920.12670771825511487No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5890.12606561833152474No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA5850.12520948510007127No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5730.12264108540571082No Hit
CTCTTACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG5630.12050075232707715No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5570.11921655247989693No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5560.11900251917203356No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5560.11900251917203356No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5550.11878848586417018No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTTCCTGTAA5490.11750428601698995No Hit
TCCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5420.11600605286194639No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5380.1151499196304929No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5370.11493588632262952No Hit
TACCCTGGAAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5290.11322361985972257No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5270.11279555324399584No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5250.11236748662826908No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5220.11172538670467898No Hit
GCAGACGCAGAAGGGGACGATAGAGTCCCAAGCGGCCGCGAGACGGATCG5160.11044118685749876No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5130.10979908693390865No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5010.1072306872395482No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4990.10680262062382148No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4960.10616052070023137No Hit
TAGACAAAGGCATTAAGCCTTATTATCCGGAACATGCAGTTAATCATTAC4930.10551842077664127No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4880.10444825423732441No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT4860.10402018762159766No Hit
CTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4840.10359212100587094No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4820.1031640543901442No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4810.10295002108228082No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4780.10230792115869071No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4780.10230792115869071No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4780.10230792115869071No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC4760.10187985454296399No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4690.1003816213879204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)