FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412729
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16800.4070467546501457No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16750.4058353059755917No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT13340.32321450637100857No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13090.31715726299823854No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12080.2926859997722477No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11440.27717945673795635No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9840.23841309915222822No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA9450.22896379949070697No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9110.22072594850373975No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8820.2136995461913265No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8790.2129726769865941No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACCTACAAGGCC8330.20182734918069725No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8240.19964674156650009No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8100.19625468527774884No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGATGGAGTTTG7900.19140889057953284No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7780.1885014137606032No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7300.17687150648488476No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6930.16790678629318512No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA6900.1671799170884527No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6730.1630609915949691No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6710.1625764121251475No Hit
ATCTTAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG6560.15894206610148548No Hit
GGTTAAGATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGAGAAAGTG6520.15797290716184229No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6360.15409627140326945No Hit
ATTTTGAGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6290.15240024325889384No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6250.15143108431925065No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6210.15046192537960745No Hit
CCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6170.14949276643996423No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT6140.14876589723523184No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6050.14658528962103462No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6030.146100710151213No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5990.14513155121156981No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG5860.1419817846577294No Hit
CTTTAATCCTGAGTGGCAAACTCCATCCTTTCCTCACATTCATTTACAGG5810.1407703359831754No Hit
GCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT5770.13980117704353218No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5750.1393165975737106No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5740.1390743078387998No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5650.1368937002246026No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5600.13568225155004857No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5500.13325935420094057No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5320.12889813897254615No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5300.12841355950272454No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5290.12817126976781373No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCCTTTCCTCAC5280.12792898003290296No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA5250.12720211082817054No Hit
CCTCCTGCTTACAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA5110.12381005453941933No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5090.12332547506959772No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5040.12211402639504372No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCCTTTC5040.12211402639504372No Hit
GTTGTCAATATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGACTGCCGTG5010.12138715719031132No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5010.12138715719031132No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4870.1179951009015601No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4860.1177528111666493No Hit
GGGTAAAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGA4830.11702594196191689No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT4830.11702594196191689No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4830.11702594196191689No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4820.1167836522270061No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4790.11605678302227368No Hit
ATGTAAGACCTTAGGCAAGACCTGGTGGGCGTGGGCGTTCACGGTGGTCT4750.1150876240826305No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4680.11339159593825487No Hit
TATTAACATTGACATAGCTGACCACTAATTCCCTGGATGCTGGGTCTTCC4660.11290701646843328No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4650.11266472673352249No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4650.11266472673352249No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC4640.1124224369986117No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGTGGGCGTGG4600.11145327805896849No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT4600.11145327805896849No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA4590.11121098832405768No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA4550.11024182938441447No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4420.10709206283057406No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT4360.10563832442110925No Hit
ATTTGGAGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA4300.10418458601164444No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4290.10394229627673365No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4290.10394229627673365No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4280.10370000654182285No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4280.10370000654182285No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4260.10321542707200124No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4200.10176168866253643No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4140.10030795025307163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)