FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412729
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16650.40341240862648375No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15460.3745799301720984No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14540.3522892745603047No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11820.2863864666645668No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT9610.2328404352492798No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9080.21999907929900736No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9080.21999907929900736No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8910.21588015380552372No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8690.2105497796374861No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8200.19867758262685684No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7930.19213575978426523No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7570.1834133293274764No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA7130.17275258099140114No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGATGGAGTTTG6960.1686336554979175No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6780.1642724402695231No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACCTACAAGGCC6550.15869977636657467No Hit
ATCTTAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG6140.14876589723523184No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6010.14561613068139143No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5920.1434355230671942No Hit
GGTTAAGATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGAGAAAGTG5810.1407703359831754No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5780.140043466778443No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5760.1395588873086214No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5550.13447080287549457No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5460.13229019526129734No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5460.13229019526129734No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5310.12865584923763537No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT5270.12768669029799215No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5220.12647524162343815No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5220.12647524162343815No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA5190.12574837241870573No Hit
CTTTAATCCTGAGTGGCAAACTCCATCCTTTCCTCACATTCATTTACAGG5180.12550608268379493No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5150.12477921347906253No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5120.12405234427433014No Hit
ATTTTGAGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA5100.12356776480450851No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5060.12259860586486533No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4920.11920654957611411No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA4910.11896425984120332No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4900.11872197010629251No Hit
GTTGTCAATATGCCCTGAGCCTGCGGGCTCCACCCCAAAAGACTGCCGTG4870.1179951009015601No Hit
GCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTT4840.1172682316968277No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4780.11581449328736289No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4730.11460304461280889No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCCTTTC4620.11193785752879007No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4590.11121098832405768No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4540.10999953964950368No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4490.10878809097494967No Hit
CCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4470.10830351150512806No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG4450.10781893203530646No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4380.10612290389093086No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4370.10588061415602004No Hit
GGGTAAAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAGGTTGGCGA4320.10466916548146604No Hit
CCTCCTGCTTACAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA4310.10442687574655524No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4290.10394229627673365No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCCTTTCCTCAC4260.10321542707200124No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4250.10297313733709043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)