FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361943
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC22970.6346303147180633No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12630.3489499727857701No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC11940.329886197550443No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11070.30584926355807407No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11060.30557297696046065No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10780.2978369522272844No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT8920.24644764507118525No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8550.23622504095948807No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8400.23208074199528658No Hit
ATACTATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7910.21854269871222817No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG7750.21412211315041318No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6820.1884274595723636No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT6700.18511202040100236No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCA6660.18400687401054863No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA6200.17129769052033056No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5900.16300909259192747No Hit
GTGTAAAAGGGGCAGCAAAGCCCAATAGACCCACAATTCTCTGACATACT5250.1450504637470541No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5120.14145873797807942No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5110.141182451380466No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5080.14035359158762567No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5070.14007730499001222No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5060.1398010183923988No Hit
GCTTCGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT5060.1398010183923988No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5040.13924844519717192No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC5010.13841958540433164No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT4840.1337227132449032No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4760.13151242046399572No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC4640.12819698129263446No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4560.125986688511727No Hit
GTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT4400.12156610294991199No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4320.11935581016900453No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4270.11797437718093734No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC4160.11493522460718952No Hit
CAATTACATATCCCATGAAATTAAGGGAGTAGCCCCAACGTTTGGTTTTA4130.11410636481434921No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4110.11355379161912234No Hit
AATTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4090.11300121842389547No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4000.11051463904537455No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC3970.10968577925253424No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT3920.10830434626446706No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3910.10802805966685362No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3910.10802805966685362No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG3820.10554148028833268No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA3800.10498890709310582No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3780.10443633389787894No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3770.10416004730026553No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3770.10416004730026553No Hit
ATGTAATTGGATGTTGGGGTACGTTACCGCAAGACCATATTGTACTAAAA3760.10388376070265208No Hit
ACCATATTGTACTAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAAAT3710.1025023277145849No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT3700.10222604111697145No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCATTAAC3670.10139718132413116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)