FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426277
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55111.2928213344843846No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9350.2193409449724006No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7940.18626386129207065No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7430.17429980974812154No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6310.1480258142006254No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6270.14708745721678626No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6150.14427238626526884No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5710.1339504594430382No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5470.12832031754000334No Hit
GGATAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5440.12761654980212397No Hit
CCCTTACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGG5400.12667819281828482No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5380.12620901432636525No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5360.12573983583444567No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA5320.12480147885060654No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5080.11917133694757166No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5010.11752921222585315No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4640.10884941012534104No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4480.10509598218998444No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4470.10486139294402466No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4460.10462680369806487No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4460.10462680369806487No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4340.10181173274654742No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT4330.10157714350058765No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4310.10110796500866807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)