FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519832
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT33200.6386678773142092No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTATTTAGTGCTGTT18450.35492235953154094No Hit
GTCTAGAGGCAAGTATTTAGTGCTGTTAGGAAAAAATCTAGCAGGCATAA18110.3483817848843473No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCCATGAA16020.3081764877883624No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC14830.28528447652318445No Hit
CTCTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT13940.26816356053494206No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13270.25527478108311913No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA13090.2518121239169578No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12740.24507917942719956No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT12740.24507917942719956No Hit
CCTCTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA12550.24142415241847365No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12240.23546068729897351No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA12120.23315224918819927No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11740.22584219517074747No Hit
GTATAATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCC11520.22161005863432803No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG11150.21449237445944075No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11110.21372289508918269No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT10830.2083365394973761No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCATTTACATG10600.20391203311839212No Hit
ATTTAGTGCTGTTAGGAAAAAATCTAGCAGGCATAATTAATTTCAGTCTT10520.2023730743778759No Hit
TCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG10080.19390880130503702No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG9630.18525215838963358No Hit
GTATTTAGTGCTGTTAGGAAAAAATCTAGCAGGCATAATTAATTTCAGTC9580.18429030917681097No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA9470.18217424090860124No Hit
CATGAAGACATTTCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT9200.17698025515935917No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG9150.17601840594653656No Hit
GGATCATATTATACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA9030.17370996783576234No Hit
GCACTAAATACTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT8460.16274488680958463No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8450.1625525169670201No Hit
GCTTTATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTT8420.16197540743932656No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC8370.16101355822650396No Hit
GTTCACATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA8280.15928222964342326No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8110.1560119423198264No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7840.15081795657058433No Hit
CCATTTACACAATGTGGTTACCCTGCTTTAATGCCTTTGTATGCATGTAT7770.1494713676726327No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTGTCCCTATC7480.1438926422382616No Hit
ATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTT7430.14293079302543898No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7430.14293079302543898No Hit
CTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAA7290.1402376152295357No Hit
CTGTAAATGAAAAGAGAAGACTGAAATTAATTATGCCTGCTAGATTTTTT7270.13985287554440665No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC7160.13773680727619694No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC7100.1365825882208098No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT7090.13639021837824528No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG7070.13600547869311624No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT6970.13408178026747103No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTG6850.13177334215669678No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6740.12965727388848705No Hit
TTTCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAG6700.128887794518229No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCA6690.12869542467566444No Hit
GTCAAGGACCACTGGCCACAAGCCAACCAGGTAGGAGTGGGAGCATTCGG6660.12811831514797087No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA6580.1265793564074547No Hit
TCCCGATACAGAGCTGAGGCTGTATCTAGTAAATCCCGGACGGAAGGAAA6560.12619461672232568No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAGAGAAGACTGAAATTAATTATG6520.1254251373520676No Hit
CAATAGGCCTATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT6500.12504039766693856No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT6460.12427091829668047No Hit
ACTAAATACTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGATCA6460.12427091829668047No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTATTTAGTG6420.12350143892642239No Hit
CTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6380.1227319595561643No Hit
CATTTCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAA6330.12177011034334169No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6230.11984641191769649No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC6210.11946167223256744No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA6100.11734560396435771No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTAT6020.11580664522384154No Hit
GTGTAGTTTCCCTCTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAA5990.115229535696148No Hit
GGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT5890.11330583727050278No Hit
CCTATTAGAAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGAGTTT5880.11311346742793825No Hit
GGGTAAAGGTTCACATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA5850.1125363579002447No Hit
TTCTAAAACAGTGTTTGATTTTTTGTATAATATGATCCTGTGGCAATGAA5780.11118976900229305No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTC5770.11099739915972852No Hit
ATATTATACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCAATAGG5690.10945844041921236No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAAGCGAAACAGGCTTTTACTTT5630.10830422136382523No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCTGTATCTAGTAAATCCCGGA5590.10753474199356715No Hit
ATACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCT5560.10695763246587359No Hit
CCCATGATGTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAGG5550.10676526262330907No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA5550.10676526262330907No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA5430.10445682451253482No Hit
GTTTTAGAAAACTTCCTGTCAATAGGCCTATTGATTGGAAAGTATGTCAG5400.10387971498484125No Hit
ATACAGCACTCAGGCAAGCCATTCTTTGCTGGGGGGAATTAATGACTCTA5330.10253312608688961No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5270.10137890703150249No Hit
ATTACATACCCCATGAAATTCAGGGAATAACCCCATCTCTTTGTTTTGTT5230.10060942766124441No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5210.10022468797611535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)