FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519832
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT28670.5515243386324813No Hit
GTCTAGAGGCAAGTATTTAGTGCTGTTAGGAAAAAATCTAGCAGGCATAA20900.4020529709598486No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTATTTAGTGCTGTT18240.350882592837686No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCCATGAA16210.3118315147970883No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15700.30202065282629775No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC15050.28951661305960386No Hit
CTCTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT14930.28720817494882966No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG14310.27528124470982934No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA13320.25623663029594174No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13210.25412056202773203No Hit
CCTCTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA12300.23661490635436064No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12130.2333446190307638No Hit
GTATAATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCC12050.2318056602902476No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA11330.21795503162560212No Hit
GTATTTAGTGCTGTTAGGAAAAAATCTAGCAGGCATAATTAATTTCAGTC11220.2158389633573924No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11210.21564659351482784No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT11110.21372289508918269No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG11060.21276104587636008No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT10630.20448914264608567No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG10310.198333307684021No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCATTTACATG10070.19371643146247247No Hit
GGATCATATTATACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA10020.19275458224964986No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9870.18986903461118207No Hit
ATTTAGTGCTGTTAGGAAAAAATCTAGCAGGCATAATTAATTTCAGTCTT9700.18659874728758521No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA9440.1815971313809077No Hit
TCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG9140.17582603610397204No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8770.1687083519290848No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC8690.1671693931885686No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC8640.166207543975746No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8020.1542806137367457No Hit
CCATTTACACAATGTGGTTACCCTGCTTTAATGCCTTTGTATGCATGTAT7860.1512026962557134No Hit
GCACTAAATACTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT7750.14908662798750366No Hit
GTTCACATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7580.1458163406639068No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCA7530.1448544914510842No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTG7260.13966050570184213No Hit
CATGAAGACATTTCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT7200.138506286646455No Hit
ATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTT7080.13619784853568076No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT7070.13600547869311624No Hit
GCTTTATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTT7010.13485125963772912No Hit
ATATTATACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCAATAGG6970.13408178026747103No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAGTATTTAGTG6880.13235045168439036No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6780.13042675325874514No Hit
GTTTTAGAAAACTTCCTGTCAATAGGCCTATTGATTGGAAAGTATGTCAG6770.1302343834161806No Hit
CTGTAAATGAAAAGAGAAGACTGAAATTAATTATGCCTGCTAGATTTTTT6750.12984964373105157No Hit
CTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAA6630.12754120562027732No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG6500.12504039766693856No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC6500.12504039766693856No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6420.12350143892642239No Hit
TTTCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAG6420.12350143892642239No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT6410.12330906908385787No Hit
TTCTAAAACAGTGTTTGATTTTTTGTATAATATGATCCTGTGGCAATGAA6370.12253958971359978No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT6340.1219624801859062No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTGTCCCTATC6310.12138537065821266No Hit
GGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT6210.11946167223256744No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC6110.11753797380692223No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6050.1163837547515351No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC6020.11580664522384154No Hit
ACTAAATACTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGATCA5910.11369057695563181No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA5890.11330583727050278No Hit
TCCCGATACAGAGCTGAGGCTGTATCTAGTAAATCCCGGACGGAAGGAAA5840.11234398805768017No Hit
ATATAGGATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAA5810.11176687852998662No Hit
GTATGTGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG5770.11099739915972852No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5750.11061265947459949No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5730.11022791978947044No Hit
CCTATTAGAAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGAGTTT5730.11022791978947044No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG5700.10965081026177688No Hit
GTCAAGGACCACTGGCCACAAGCCAACCAGGTAGGAGTGGGAGCATTCGG5630.10830422136382523No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAGAGAAGACTGAAATTAATTATG5520.10618815309561551No Hit
CATTTCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAA5520.10618815309561551No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAAGCGAAACAGGCTTTTACTTT5500.10580341341048646No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTC5480.10541867372535743No Hit
ATACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCT5390.10368734514227675No Hit
GTGTAGTTTCCCTCTTATATAGGATCCCAGCCTTCCAAAGAGTATGTAAA5390.10368734514227675No Hit
AAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGGATAATA5380.10349497529971222No Hit
CTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5360.10311023561458317No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5350.10291786577201864No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA5340.10272549592945414No Hit
CAATAGGCCTATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT5310.10214838640176056No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA5240.10080179750380892No Hit
ATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCATTTACA5230.10060942766124441No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA5220.10041705781867988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)