FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375485
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAAAGTCCAA15200.404809779352038No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT14070.3747153681238931No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13110.3491484346911328No Hit
ACTACATACTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA10920.29082386779764835No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10400.2769751121882365No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9570.25487036765782917No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9440.2514081787554762No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9350.24901127874615495No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAA9220.245549089843802No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8100.21572100083891502No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7960.21199248971330412No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7950.2117261674900462No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7550.20107327855972942No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7350.195746834094571No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT7350.195746834094571No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7170.19095303407592848No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7140.1901540674061547No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6900.18376233404796463No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTGAGA6680.17790324513629038No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6640.1768379562432587No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6490.17284312289438994No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6460.17204415622461616No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACATTGCCACAG6340.16884828954552114No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT6180.1645871339733944No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6160.16405448952687857No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6080.16192391174081522No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTGAGACA6010.1600596561780098No Hit
GTCATTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5800.15446688948959347No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5760.15340160059656177No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5740.15286895615004595No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATACCCACATTGCGTGAAA5720.1523363117035301No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5620.1496730894709509No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACA5540.14754251168488755No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5540.14754251168488755No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA5460.14541193389882417No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATT5440.14487928945230835No Hit
ATATTAACGTTGACATAGCTGACCACTAATTCCCTGGATGCTGGATCTTC5270.14035181165692373No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5170.1376885894243445No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5060.1347590449685074No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5050.13449272274524948No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5020.1336937560754757No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4900.13049788939638068No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4870.1296989227266069No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4830.12863363383357523No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4810.1281009893870594No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4780.12730202271728563No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4760.1267693782707698No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTC4750.12650305604751189No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTGTC4720.1257040893777381No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4680.12463880048470645No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT4680.12463880048470645No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4670.12437247826144852No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4550.1211766115823535No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA4530.12064396713583765No Hit
GCTCAGAACACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4520.12037764491257973No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4500.11984500046606389No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4490.11957867824280598No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4480.11931235601954804No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4480.11931235601954804No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4450.11851338934977429No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC4420.11771442268000054No Hit
CCTTTATGCCGCTGTTACCCATTTTCTTTTGTCTTTGGGTATACATTTAA4400.1171817782334847No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4370.11638281156371093No Hit
CTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGGAGAGAAACTGTTCT4320.11505120044742133No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4290.11425223377764758No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA4260.1134532671078738No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4260.1134532671078738No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT4250.1131869448846159No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4230.11265430043810006No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4230.11265430043810006No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG4230.11265430043810006No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA4200.1118553337683263No Hit
ATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4190.11158901154506838No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4170.11105636709855253No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4170.11105636709855253No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT4140.11025740042877878No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4130.10999107820552086No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4120.10972475598226294No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4100.1091921115357471No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4090.1089257893124892No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4030.10732785597294167No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4020.10706153374968373No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3990.10626256707990998No Hit
TTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGAGACATTATT3990.10626256707990998No Hit
CATATAAAGGCATTAAAGCAGGATACCCACATTGCGTGAAAGGGGCGGCA3990.10626256707990998No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3960.10546360041013624No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3930.10466463374036246No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTG3900.10386566707058871No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3880.10333302262407287No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA3850.10253405595429911No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3810.10146876706126744No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3800.1012024448380095No Hit
GGGTAAAGGTTTCGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3780.10066980039149366No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC3770.10040347816823575No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3760.10013715594497782No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC3760.10013715594497782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)