FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375485
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAAAGTCCAA14510.3864335459472416No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13720.3653940903098659No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT13180.3510126902539382No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9570.25487036765782917No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9370.24954392319267082No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9270.24688070096009163No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAA9140.24341851205773868No Hit
ACTACATACTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA8600.22903711200181098No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8560.2279718231087793No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8280.22051480085755756No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8160.21731893417846251No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7720.20560075635511404No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTGAGA7420.19761108965737645No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7240.1928172896387339No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7160.19068671185267055No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7060.18802348962009136No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6800.18109911181538543No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT6750.17976750069909583No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6660.17737060068977456No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTT6640.1768379562432587No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6330.16858196732226322No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6310.16804932287574736No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6260.16671771175945777No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6160.16405448952687857No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6090.16219023396407312No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6070.1616575895175573No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACATTGCCACAG5980.15926068950823602No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATACCCACATTGCGTGAAA5950.15846172283846227No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA5600.14914044502443508No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5490.14621090056859795No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5410.14408032278253458No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5330.14194974499647123No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5310.14141710054995538No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5290.14088445610343955No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5280.14061813388018163No Hit
GCTCAGAACACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5150.13715594497782868No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5040.13422640052199156No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTGAGACA5030.13396007829873366No Hit
ATATTAACGTTGACATAGCTGACCACTAATTCCCTGGATGCTGGATCTTC4960.13209582273592818No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCCATTTTCTTTTGTC4920.13103053384289653No Hit
GTCATTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4900.13049788939638068No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4880.12996524494986483No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4840.12889995605683316No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATT4780.12730202271728563No Hit
CCTTTATGCCGCTGTTACCCATTTTCTTTTGTCTTTGGGTATACATTTAA4740.12623673382425396No Hit
GGGTAAAGGTTTCGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4700.1251714449312223No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4660.12410615603819061No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACA4650.12383983381493269No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4600.12250822269864309No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4530.12064396713583765No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4510.12011132268932181No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4490.11957867824280598No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4430.11798074490325845No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4380.11664913378696885No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4270.11371958933113174No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4250.1131869448846159No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4250.1131869448846159No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4240.11292062266135797No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4200.1118553337683263No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4190.11158901154506838No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4180.11132268932181047No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4140.11025740042877878No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG4130.10999107820552086No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4120.10972475598226294No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4120.10972475598226294No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4110.109458433759005No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4110.109458433759005No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4080.10865946708923126No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT4060.10812682264271542No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4050.10786050041945751No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4040.10759417819619958No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4030.10732785597294167No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4020.10706153374968373No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC4020.10706153374968373No Hit
ATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4020.10706153374968373No Hit
CTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGGAGAGAAACTGTTCT4010.10679521152642582No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATACTCTGG3990.10626256707990998No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3990.10626256707990998No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3950.1051972781868783No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3920.10439831151710455No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3900.10386566707058871No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3870.10306670040081493No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3860.10280037817755702No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT3840.10226773373104119No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3830.10200141150778327No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3800.1012024448380095No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3790.1009361226147516No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3770.10040347816823575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)