FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599146
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN74931.2506133730342854No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG30550.5098924135352653No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG18550.3096073411155211No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT17640.2944190564570238No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG16300.27205389003681907No Hit
ATCCTAATCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT14770.2465175433033017No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGTTGACATACT14750.2461837348492688No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT13940.23266449246093607No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13870.2314961628718209No Hit
GATTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG13390.22348475997503114No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGATT13220.2206473881157514No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12530.20913099645161615No Hit
GTTCTATCCTAATCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC12310.20545910345725416No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11680.19494413715521758No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11460.19127224416085561No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11030.18409536239914814No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10500.1752494383672761No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA10040.16757184392451924No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA10020.16723803547048632No Hit
GGTTTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG9830.1640668551571737No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG9800.16356614247612436No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC9720.16223090865999273No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC9530.1590597283466801No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT9480.15822520721159786No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT9370.15638926071441686No Hit
GGGTAAAGGTTTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9280.15488712267126878No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA9080.1515490381309397No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9070.15138213390392324No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG9040.1508814212228739No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8980.14987999586077516No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGGCAGGTCTTTGCCAAGTG8800.14687571977447902No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8620.14387144368818286No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8430.14070026337487024No Hit
GCCTAGAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTAT8370.13969883801277153No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8260.13786289151559053No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7770.1296845843917843No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7670.12801554212161978No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7640.1275148294405704No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7620.1271810209865375No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA7500.12517817026234007No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7420.12384293644620843No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7310.12200698994902744No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7240.12083866035991228No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC7230.12067175613289582No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7190.12000413922483No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6830.11399558705223768No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6800.11349487437118833No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG6790.11332797014417188No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6680.11149202364699089No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA6610.1103236940578757No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6400.10681870529053018No Hit
ATCTAAGGGCAAATATTTGGTACGATTAGGATAGAACCTAGCAGGCATAA6400.10681870529053018No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG6320.10548347147439854No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA6290.1049827587933492No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT6210.10364752497721756No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6140.1024791953881024No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6090.10164467425302012No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6090.10164467425302012No Hit
CTTTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6070.10131086579898722No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6040.10081015311793787No Hit
ATGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACACAAA6010.10030944043688851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)