FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532176
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA23350.43876461922371546No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGCAAATAATGTCGGGTTT19870.37337271879979556No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14750.2771639457623042No Hit
CTCAAGGACAGTGTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA14520.2728420672860106No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13980.26269504825471274No Hit
GGATAATAAGGTTTAATCCCCTTGTCTAGAGGCAAGTATTTAGTGCTGTT13220.2484140585069601No Hit
CTATAGGCCTATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT13040.2450317188298608No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGGTATTCCCTGAATTTCAT12300.2311265446017859No Hit
GTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA12160.22849583596404197No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC12070.22680466612549233No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT11200.21045669101951234No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG10940.20557108926370227No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA10780.20256456510628065No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10730.2016250263070864No Hit
GTCTAGAGGCAAGTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA10630.19974594870869788No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG10440.1961757012717597No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAA10330.19410871591353235No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG10290.19335708487417697No Hit
CCATTTACACAATGTGGTTACCCTGCCTTAATGCCTTTGTATGCATGTAT9670.18170680376416826No Hit
GTGTAAATGGAGCAGCAAAGCCCAAGAGACCCACAATTCTCTGACATACT9640.1811430804846517No Hit
CCTCTAGACAAGGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA9620.180767264964974No Hit
TCCAAAGAGTATGCAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA9540.1792640028862632No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGCAAATAATGTCGGGT9470.17794864856739123No Hit
GTCCTTGAGTATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9420.17700910976819698No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC9170.17231141577222572No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCATTTACATG9060.1702444304139984No Hit
GTATAAAGGGACTCCAGATGTTGTACAGACTTGGCCCCCAGTACCACATC8990.16892907609512642No Hit
CCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTA8630.1621643967409278No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCCATGAA8560.16084904242205586No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTGTCTCTTCCAAAAGTAAGG8540.16047322690237814No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTGGAGTCCCTTTATACC8440.15859414930398966No Hit
GGCCTATAGATTGGAAAGTATGTCAGAGAATTGTGGGTCTCTTGGGCTTT8340.15671507170560117No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT8200.15408436306785725No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA8180.15370854754817956No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC7880.14807131475301405No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACAGCACTCAGGCA7640.14356152851688164No Hit
GGATCATATTCTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA7550.14187035867833198No Hit
ATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAGTCTT7460.14017918883978234No Hit
ATACAGCACTCAGGCAAGCCATTCTTTGCTGGGGGGAATTAATGACTCTA7140.1341661405249391No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT6980.13115961636751752No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6760.1270256456510628No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTG6670.12533447581251314No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6670.12533447581251314No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACAAAGCTCTGCAAG6670.12533447581251314No Hit
TTCTAAAACAGTGTTTGATTTTTTGTAGAATATGATCCTGTGGCAATGAA6560.1232674904542858No Hit
GGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT6420.12063678181654189No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6420.12063678181654189No Hit
GCACTAAATACTTGCCTCTAGACAAGGGGATTAAACCTTATTATCCTGAT6360.1195093352575088No Hit
CATTTCTACTAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAA6310.11856979645831454No Hit
GTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAGTC6310.11856979645831454No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTGTCCCTATC6250.11744234989928144No Hit
TCCCGATACAGAGCTGAGGCCGTATCTAGTAGATCCCGGACGGAAGGAAA6240.11725444213944258No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT6150.11556327230089294No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACAG6090.11443582574185984No Hit
GGGTAAAGGTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA6090.11443582574185984No Hit
AGTCTGTACAACATCTGGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6070.11406001022218214No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG6040.11349628694266559No Hit
TCACCATACAGCACTCAGGCAAGCCATTCTTTGCTGGGGGGAATTAATGA6040.11349628694266559No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5980.11236884038363248No Hit
GCCTTAATGCCTTTGTATGCATGTATTCAAGCGAAACAGGCTTTTACTTT5960.11199302486395478No Hit
CTGTAAATGAAAAGAGAAGACTGAAATTAATTATGCCTGCTAGATTTTAT5880.11048976278524399No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCA5820.10936231622621088No Hit
ATACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCT5810.10917440846637202No Hit
GGTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5790.10879859294669432No Hit
CTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAA5760.10823486966717777No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5750.10804696190733892No Hit
ATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTT5740.10785905414750008No Hit
GCTTTATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTT5640.10597997654911158No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5620.10560416102943387No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA5610.10541625326959503No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCCGTATCTAGTAGATCCCGGA5610.10541625326959503No Hit
CTACTAGATACGGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5590.10504043774991732No Hit
CATCTGGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGG5590.10504043774991732No Hit
GTACGGCAGACGGAGAAGGGGACGATAGAGCCCCAAGCGACCCCGCGAAG5500.10334926791136767No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT5470.10278554463185113No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT5470.10278554463185113No Hit
GGATTAAAGATAGGGACAGTAGAGGAATAAAGCCCAGTAAAATTTCCCAC5330.10015483599410721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)