FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532176
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGCAAATAATGTCGGGTTT21210.3985523586182015No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18530.3481930789813896No Hit
GGATAATAAGGTTTAATCCCCTTGTCTAGAGGCAAGTATTTAGTGCTGTT14240.26758065001052284No Hit
CTCAAGGACAGTGTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA13830.25987643185713No Hit
GTCTAGAGGCAAGTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA13350.2508568593848652No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13270.24935359730615436No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13040.2450317188298608No Hit
GTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA12690.23845494723550104No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGGTATTCCCTGAATTTCAT12420.23338143771985206No Hit
CTATAGGCCTATTGACAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT12310.23131445236162473No Hit
TTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC12190.22905955924355853No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG12180.22887165148371968No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG11550.2170334626138721No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGCAAATAATGTCGGGT11210.2106445987793512No Hit
GTGTAAATGGAGCAGCAAAGCCCAAGAGACCCACAATTCTCTGACATACT10700.20106130302756983No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10670.20049757974805327No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA10510.19749105559063165No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAA10050.18884729863804456No Hit
GTATAAAGGGACTCCAGATGTTGTACAGACTTGGCCCCCAGTACCACATC9920.1864044977601395No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT9840.1849012356814287No Hit
GTCCTTGAGTATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9700.1822705270436848No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCATTTACATG9290.17456630889029193No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTGTCTCTTCCAAAAGTAAGG9140.1717476924927092No Hit
TCCAAAGAGTATGCAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA9100.17099606145335378No Hit
CCCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTA9090.17080815369351493No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8750.164419289858994No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTGGAGTCCCTTTATACC8490.1595336881031839No Hit
CCATTTACACAATGTGGTTACCCTGCCTTAATGCCTTTGTATGCATGTAT8490.1595336881031839No Hit
GTAGAGGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT8350.15690297946544No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATACCCCATGAA8100.15220528546946874No Hit
ATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAGTCTT8020.15070202339075794No Hit
GGCCTATAGATTGGAAAGTATGTCAGAGAATTGTGGGTCTCTTGGGCTTT7990.1501383001112414No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC7960.14957457683172484No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7830.1471317759538198No Hit
CCTCTAGACAAGGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA7780.14619223715462554No Hit
GTATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTCAGTC7630.14337362075704277No Hit
GGATCATATTCTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA7450.1399912810799435No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACAGCACTCAGGCA7420.13942755780042693No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7030.13209915516671178No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT6770.12721355341090165No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAGCTTTCCACAAAGCTCTGCAAG6720.1262740146117074No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT6660.1251465680526743No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACAG6550.12307958269444695No Hit
ATACAGCACTCAGGCAAGCCATTCTTTGCTGGGGGGAATTAATGACTCTA6510.12232795165509154No Hit
GGGTAAAGGTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA6490.12195213613541385No Hit
TTCTAAAACAGTGTTTGATTTTTTGTAGAATATGATCCTGTGGCAATGAA6430.12082468957638073No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC6220.1168786266197649No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG6110.11481164126153753No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT6100.11462373350169869No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGCAAA6080.11424791798202098No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT6060.11387210246234329No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG6060.11387210246234329No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTG6050.11368419470250443No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6050.11368419470250443No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCA6030.11330837918282673No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6020.11312047142298788No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCCGTATCTAGTAGATCCCGGA6010.11293256366314904No Hit
AGTCTGTACAACATCTGGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5920.11124139382459937No Hit
GCCTTAATGCCTTTGTATGCATGTATTCAAGCGAAACAGGCTTTTACTTT5840.10973813174588858No Hit
GGTTCATATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5750.10804696190733892No Hit
ATACTAACATGGGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCT5650.10616788430895041No Hit
GCACTAAATACTTGCCTCTAGACAAGGGGATTAAACCTTATTATCCTGAT5620.10560416102943387No Hit
GGCCTAAAGATCAGGCAACTATTGTGGTTTCATATTTCTTGCCTTACTTT5590.10504043774991732No Hit
TCCCGATACAGAGCTGAGGCCGTATCTAGTAGATCCCGGACGGAAGGAAA5590.10504043774991732No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA5580.10485252999007848No Hit
GCTTTATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTT5550.10428880671056193No Hit
CTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAA5540.10410089895072307No Hit
ATTCCTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTT5540.10410089895072307No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA5470.10278554463185113No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCCTCTACTGTCCCTATC5390.10128228255314033No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC5340.10034274375394607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)