FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427676
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30260.7075449639446684No Hit
GTTTATAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT18090.4229837540568094No Hit
AGTCTATACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT12700.29695376874082247No Hit
CCTATAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT12330.28830235973026314No Hit
CTCTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT11580.27076571984399406No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11350.2653878169455382No Hit
AAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGATTTAAATGTAT10020.23428950888055444No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC9150.21394700661248237No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT9110.21301171915188133No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATTATT8350.19524125740046203No Hit
ATATGTTCCTGTGGCAATGAGCCCCAACTCCCAATTACATATCCCATGAA8050.1882266014459544No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA7870.18401780787324984No Hit
CTCCAAAAGACACCAGATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7860.1837839860080996No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT7550.17653550818844171No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACA7220.16881938663848334No Hit
CATTTAAATCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT7090.16577970239153003No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6950.16250619627942647No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6780.15853122457187216No Hit
ATATGGTATTGGGGGCCAAGTCTATACAACATCTTGAGTCCCTTTATGCC6750.1578297589764214No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6390.14941217183101227No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT6360.14871070623556149No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6340.148243062505261No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6190.1447357345280072No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6070.14192987214620414No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA6020.14076076282045288No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA5920.13842254416895033No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC5840.13655196924774832No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA5650.13210935380989347No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5500.12860202583263966No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT5500.12860202583263966No Hit
CTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTTT5470.1279005602371889No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA5440.12719909464173812No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC5370.12556234158568635No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTTTGGA5270.12322412293418383No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5270.12322412293418383No Hit
CCAAAAGACACCAGATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5120.11971679495693002No Hit
CCCTTAATTTCATGGGATATGTAATTGGGAGTTGGGGCTCATTGCCACAG5110.11948297309177976No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5090.11901532936147924No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5080.118781507496329No Hit
GTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGG5060.11831386376602848No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT5030.11761239817057773No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5000.11691093257512697No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA4980.11644328884482646No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTATAA4960.11597564511452595No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4920.11504035765392494No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG4910.11480653578877467No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4840.11316978273272289No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4830.11293596086757265No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4720.11036392035091987No Hit
CTCTCTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTC4690.10966245475546908No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT4670.10919481102516859No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG4490.10498601745246401No Hit
AAATGAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGATTTAAATGTAT4450.104050729991863No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATATTCAA4450.104050729991863No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4440.10381690812671274No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4420.10334926439641223No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGGAAAGAAGGAGTTT4410.10311544253126198No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4410.10311544253126198No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT4350.10171251134036047No Hit
GTATAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGA4350.10171251134036047No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4280.10007575828430869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)