FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427676
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30190.7059082108886167No Hit
GTTTATAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT16930.3958604176993799No Hit
AGTCTATACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT14730.34441960736632404No Hit
CCTATAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT14060.32875354240125704No Hit
CTCTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT11780.27544215714699916No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10650.24902028638502044No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC9700.22680720919574632No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT9320.21792197832003665No Hit
ATATGGTATTGGGGGCCAAGTCTATACAACATCTTGAGTCCCTTTATGCC8930.20880292557917673No Hit
ATATGTTCCTGTGGCAATGAGCCCCAACTCCCAATTACATATCCCATGAA8380.19594272299591278No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAAACGAGACATTATT8280.19360450434441026No Hit
AAATTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGATTTAAATGTAT8110.18962953263685595No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT8000.18705749212020314No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT7860.1837839860080996No Hit
CTCCAAAAGACACCAGATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7760.18144576735659707No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7620.1781722612444935No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT7240.16928703036878384No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC6950.16250619627942647No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACA6660.1557253621900691No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6450.15081510302191378No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6410.14987981556131277No Hit
GTATGTAAATAATGTCTCGTTTGGAAGTAATGATTAACTACATGCTCTGG6280.14684013131435947No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA6250.1461386657189087No Hit
CATTTAAATCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT6070.14192987214620414No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6030.1409945846856031No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5950.1391240097644011No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA5750.13444757246139602No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA5730.1339799287310955No Hit
TCCCATCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGG5730.1339799287310955No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5650.13210935380989347No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5600.1309402444841422No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5560.13000495702354117No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5420.12673145091143764No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA5410.12649762904628736No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA5400.12626380718113714No Hit
GTATTGGGGGCCAAGTCTATACAACATCTTGAGTCCCTTTATGCCGCTGT5230.12228883547358281No Hit
CTATACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG5230.12228883547358281No Hit
CCCTTAATTTCATGGGATATGTAATTGGGAGTTGGGGCTCATTGCCACAG5190.12135354801298179No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5140.12018443868723051No Hit
CCAAAAGACACCAGATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4920.11504035765392494No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4890.11433889205847417No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTTTGGA4860.11363742646302341No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTCC4750.11106538594637062No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT4740.11083156408122036No Hit
CTCTCTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTC4640.10849334542971782No Hit
GTATAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCCAGA4640.10849334542971782No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAGATATTCAA4630.10825952356456757No Hit
GTCTATACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTT4600.10755805796911681No Hit
CCCTTCATTTCATGGGATATGTAATTGGGAGTTGGGGCTCATTGCCACAG4590.10732423610396655No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG4510.10545366118276452No Hit
CTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTTT4460.10428455185701326No Hit
CCTGTAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4390.10264779880096148No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4320.10101104574490968No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTATAA4310.10077722387975944No Hit
ATACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4300.10054340201460919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)