FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473506
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18550.39175849936431645No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11580.24455867507486706No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGTCCCACAATTCTTTGACATACT10060.21245770908921957No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT9990.21097937512935422No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8960.18922674686276414No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG8240.1740210261327206No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7290.15395792239169093No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGACT6780.14318720354124342No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6780.14318720354124342No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6650.14044172618720777No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG6570.13875220166164737No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6540.13811862996456223No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6410.1353731526105266No Hit
CATTTAAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6330.1336836280849662No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG5540.11699957339505729No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG5290.11171980925268107No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5050.10665123567599989No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5030.10622885454460979No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5030.10622885454460979No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4980.10517290171613454No Hit
CTATTATGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT4980.10517290171613454No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGACT4930.10411694888765928No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4910.1036945677562692No Hit
CTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT4890.1032721866248791No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC4820.10179385266501374No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4770.10073789983653851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)