FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771964
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN97081.2575715966029504No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG35300.4572752097248058No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT25480.33006720520646043No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT24600.3186677098932075No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG20660.2676290604225068No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG20480.2652973454720686No Hit
TTATTAGGGTTTAAATGTATACCCAAAGATAAAAGAAAATTGGTAATAGA20480.2652973454720686No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT20100.26037483613225487No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT18190.23563274971371723No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG17980.23291241560487275No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTATC16380.21218606048986738No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT16130.2089475675031478No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA15490.2006570254571457No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC15350.1988434693845827No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14670.1900347684607054No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14070.1822623852925784No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA13600.1761740184775456No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA13570.17578539931913925No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT13250.17164012829613814No Hit
CTCCAAACGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12910.16723577783419952No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG12080.15648398111829048No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG11930.15454088532625873No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA11770.1524682498147582No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11640.150784233461664No Hit
TTATGAGGGTTTAAATGTATACCCAAAGATAAAAGAAAATTGGTAATAGA11630.15065469374219523No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC11290.1462503432802566No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC11270.14599126384131902No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11020.14275277085459942No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10970.14210507225725552No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA10610.1374416423563793No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA10530.13640532460062904No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT10450.13536900684487876No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA10330.1338145302112534No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10310.13355545077231581No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC10020.12979879890772109No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC10010.12966925918825228No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG9880.1279852428351581No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT9810.12707846479887663No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG9760.1264307662015327No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA9670.12526490872631366No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA9640.1248762895679073No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9640.1248762895679073No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC9500.12306273349534434No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT9230.11956516106968718No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA9200.11917654191128083No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9160.11865838303340571No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9010.11671528724137394No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8750.11334725453518558No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8590.11127461902368503No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC8560.1108859998652787No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTATCTTTGGGTATACATTTAAA8400.10881336435377814No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA8370.10842474519537182No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8340.10803612603696545No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGGA8230.10661118912280883No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT8170.10583395080599614No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG8150.10557487136705858No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTG8000.10363177557502681No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACT7890.1022068386608702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)