FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669834
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG32860.49056930523084824No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT31260.46668279006440405No Hit
ACTACATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA28590.4268221678804003No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT28020.41831259685235445No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTACATGTTCAGG27900.41652110821487115No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27830.4154760731763392No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT22240.33202256081357473No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG19460.29051974071187786No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG18820.2809651346453002No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC18470.2757399594526405No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA18420.27499350585368915No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC18380.27439634297452803No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA16870.25185344428619627No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG16770.25036053708829353No Hit
ATTTATAGGCAGTTTCCGAAAACATTGTTTGATTTTCTGTACAATATGTT16110.2405073495821353No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT15740.23498359294989507No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG15600.2328935228728312No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC15530.23184848783429926No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTACATGTTCA14890.22229388176772158No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTACA14820.2212488467291896No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14690.21930806737191605No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC14660.21886019521254518No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC14370.21453076433862717No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAGAGTCCAA14360.2143814736188369No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC13820.20631977475016197No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13570.20258750675540507No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC13310.1987059480408579No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT13200.19706375012316485No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG12990.19392864500756904No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA12730.19004708629302186No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG12590.187957016215958No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT12420.18541907397952329No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG12240.1827318410232983No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11470.17123645559944703No Hit
GGAACGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT11440.1707885834400762No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG11150.16645915256615818No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT11140.16630986184636792No Hit
CAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCT10840.1618311402526596No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10740.16033823305475683No Hit
TACAAGGCCTTTCTGTATAAACAATATCTGAACCTTTACCCCGTTGCTCG10310.15391873210377496No Hit
ATACGTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA10230.15272440634545276No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA9650.14406554459761672No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9620.14361767243824589No Hit
GTCGTTGACAGTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTAGG9580.1430205095590848No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT9540.14242334667992368No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA9530.1422740559601334No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG9480.14152760236118203No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9400.14033327660285982No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC9110.1360038457289418No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9010.13451093853103904No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8820.13167441485502376No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT8700.12988292621754047No Hit
GGCCCTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATG8660.12928576333837938No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC8510.12704640254152522No Hit
CTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTA8420.12570278606341273No Hit
GGAACATATTGTACAGAAAATCAAACAATGTTTTCGGAAACTGCCTATAA8410.12555349534362245No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC8340.12450846030509052No Hit
GCCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC8240.12301555310718776No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAA8180.1221198087884461No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8050.12017902943117251No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT8020.11973115727180167No Hit
GGAAAGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT8000.11943257583222112No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG7930.11838754079368918No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7870.11749179647494754No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT7850.11719321503536698No Hit
ACATATAGTTAATCATTACTTCCAAACTAGACATTATTTACATACTCTGT7770.11599888927704477No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7770.11599888927704477No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT7760.11584959855725449No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA7740.11555101711767392No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7730.11540172639788367No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7580.11316236560102952No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7570.11301307488123924No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC7570.11301307488123924No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA7520.11226662128228784No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7470.11152016768333646No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG7470.11152016768333646No Hit
GTTAGTATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTC7450.11122158624375593No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7430.11092300480417536No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG7400.11047513264480455No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA7320.10928080688648231No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7280.10868364400732121No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7190.10734002752920872No Hit
CCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7110.10614570177088652No Hit
CCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGG7080.10569782961151568No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG7030.10495137601256432No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTG7020.10480208529277403No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA6940.10360775953445181No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA6910.10315988737508099No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA6880.10271201521571016No Hit
CTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA6850.10226414305633932No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6850.10226414305633932No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG6850.10226414305633932No Hit
GTACTGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTT6820.1018162708969685No Hit
TACCGTGGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6740.1006219451386463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)