FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences669834
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTACATGTTCAGG31840.4753416518122401No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG30760.45921825407489014No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT29180.4356303203480265No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27710.41368458453885587No Hit
ACTACATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA27580.41174380518158227No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT25240.37680977675065763No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT23300.347847377111344No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG18570.27723286665054325No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG17740.2648417369079503No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA17700.26424457402878926No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC16800.25080840924766434No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA16340.24394103613731163No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC16210.24200025678003806No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTACATGTTCA16170.24140309390087694No Hit
ATTTATAGGCAGTTTCCGAAAACATTGTTTGATTTTCTGTACAATATGTT15790.23573004654884644No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15510.2315499063947187No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT15350.2291612548780743No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG15260.2278176383999618No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC15220.22722047552080066No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA15180.2266233126416396No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTACA14830.22139813744897988No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC14560.21736728801464242No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC14400.214978636497998No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC14060.20990275202512865No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA13940.2081112633876453No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13810.2061704840303717No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT13220.1973623315627454No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG12450.18586694613889412No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG12450.18586694613889412No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC12380.18482191110036217No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAGAGTCCAA11950.1784024101493803No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT11810.17631234007231641No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11560.17258007207755952No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG11360.16959425768175398No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG11170.16675773400573873No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA11140.16630986184636792No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT10550.15750170937874158No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT10450.15600880218083882No Hit
GGAACGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT10080.1504850455485986No Hit
ATACGTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA9820.14660348683405142No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9790.14615561467468058No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG9780.14600632395489033No Hit
TACAAGGCCTTTCTGTATAAACAATATCTGAACCTTTACCCCGTTGCTCG9390.14018398588306955No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT9390.14018398588306955No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC9300.13884036940495706No Hit
GCCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC9250.13809391580600566No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9230.1377953343664251No Hit
CAGGAAGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCT9170.13689959004768346No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT8970.13391377565187793No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT8950.13361519421229737No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8910.13301803133313625No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA8870.13242086845397516No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA8730.13033079837691128No Hit
GGAACATATTGTACAGAAAATCAAACAATGTTTTCGGAAACTGCCTATAA8530.12734498398110575No Hit
CTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTA8360.12480704174467107No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC8330.12435916958530024No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAA8060.12032832015096279No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA8030.11988044799159195No Hit
GGCCCTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATACATG8010.1195818665520114No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8010.1195818665520114No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8000.11943257583222112No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7980.11913399439264057No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG7830.1168946335957864No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC7720.11525243567809339No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7710.11510314495830311No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7710.11510314495830311No Hit
CCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7530.11241591200207814No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA7450.11122158624375593No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7440.11107229552396565No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7430.11092300480417536No Hit
GGAAAGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT7400.11047513264480455No Hit
CCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGG7380.11017655120522397No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT7280.10868364400732121No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTG7280.10868364400732121No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG7250.10823577184795038No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA7230.10793719040836983No Hit
ATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTACATGTTCAGGAT7180.10719073680941844No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG7170.10704144608962819No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA7130.10644428321046706No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7100.10599641105109624No Hit
CTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA7100.10599641105109624No Hit
GTCTCCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGCACT7100.10599641105109624No Hit
GTCTACGTCCCCTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7080.10569782961151568No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG6930.10345846881466154No Hit
GTCGTTGACAGTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTAGG6920.10330917809487127No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6910.10315988737508099No Hit
GGTCTATTTATAGGCAGTTTCCGAAAACATTGTTTGATTTTCTGTACAAT6890.10286130593550044No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6880.10271201521571016No Hit
GTACTGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTT6860.1024134337761296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)