FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585143
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG25860.4419432514786984No Hit
TCTATGGAGTTAATCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG19310.33000480224492135No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT16860.2881346952796154No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG16690.28522942255141054No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16380.279931572282331No Hit
CCTTTCTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCGGG16360.27958977549077746No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG15920.2720702460766001No Hit
GTTTTAGTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC15070.2575438824355756No Hit
TAGATAAAGGCATTAAACCTTATTATCCTGAACACATAGTTAATCATTAC14730.2517333369791658No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14730.2517333369791658No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG14710.25139154018761223No Hit
ATGCCGGGTAACCACATTGTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCC14400.24609368991853273No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC14170.2421630268156673No Hit
ACCCAGCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTC13270.226782171195759No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13130.22438959365488437No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC13120.22421869525910762No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA12940.22114252413512592No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT11720.20029291985036138No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11290.19294428883196074No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10860.1855956578135601No Hit
TGTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT10830.18508296262622984No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10570.1806396043360341No Hit
CCCTTTACACAATGTGGTTACCCGGCATTGATGCCTTTATATGCATGTAT10430.17824702679515947No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG10410.17790523000360595No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10350.1768798396289454No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10340.17670894123316866No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA9820.16782222465277719No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9610.16423335834146524No Hit
TCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9510.16252437438369766No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9480.1620116791963674No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC9230.1577392193019484No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG9180.1568847273230646No Hit
ACCCAGCATTAATGCCTTTATATGCATGTATACAAGCCAAGCAGGCTTTC9080.15517574336529702No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9020.15415035299063648No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8980.15346675940752944No Hit
CCGAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT8950.15295406422019917No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8930.15261226742864564No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA8870.1515868770539851No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC8790.150219689887771No Hit
CTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA8740.14936519790888725No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8700.1486816043257802No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8650.14782711234689638No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGAGAAAGGCATTAAACCTTAT8490.14509273801446826No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8490.14509273801446826No Hit
TTGTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT8480.1449218396186915No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCCGCTAGG8450.1444091444313612No Hit
CCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG8280.14150387170315631No Hit
CAGAACAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG8260.1411620749116028No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8190.1399657861411655No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA8160.1394530909538352No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8070.1379150053918444No Hit
GGTTAGGATAGAACCTAGCGGGCATAATCAATTTTAATCTCCTTTTTTCG7900.13500973266363947No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7810.13347164710164866No Hit
GTATCAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7790.13312985031009514No Hit
GTATAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7780.1329589519143184No Hit
AGTTAGAGGACAAGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT7780.1329589519143184No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7570.12937008560300645No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7560.1291991872072297No Hit
GGTTAGGATAGAACCTAGCGGGCATAATCAATTTTAATCTCCGTTTTTCG7530.1286864920198994No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7460.1274902032494621No Hit
AACCGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTT7410.1266357112705783No Hit
CATTTAAACCCTGCCAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT7300.12475582891703395No Hit
CTGCCAAAACTAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA7270.12424313372970369No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATCAATGCCGGGTAACCACAT7210.12321774335504314No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7180.12270504816771284No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT7140.12202145458460581No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6930.11843258827329388No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGGTGGCC6930.11843258827329388No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6880.1175780962944101No Hit
GTATACATGCATATAAAGGCATCAATGCCGGGTAACCACATTGTGTAAAG6880.1175780962944101No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACGGTTAACG6850.1170654011070798No Hit
AAGCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTAT6810.11638180752397277No Hit
CTTTCTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCGGGC6790.11604001073241925No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6720.11484372196198195No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT6720.11484372196198195No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT6690.11433102677465166No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA6640.11347653479576787No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6640.11347653479576787No Hit
GCTTAGCTTGTATACATGCATATAAAGGCATTAATGCTGGGTAACCACAT6500.11108395725489326No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6430.10988766848445594No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6400.10937497329712566No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6390.10920407490134891No Hit
GTTTTGGCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAC6340.1083495829224651No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6310.10783688773513483No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6290.10749509094358133No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6240.10664059896469752No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6150.1051025134027067No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTG6130.10476071661115317No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT6110.10441891981959965No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6060.10356442784071584No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6050.1033935294449391No Hit
GGGTAAAGGTTCAGATATTGTTTATACAGAAAGGCCTTGTAAGTTGGCGA6030.10305173265338559No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6030.10305173265338559No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCCGCTAGGTT5960.10185544388294829No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTCTCTAAGGGCAAATATTTGGTA5950.10168454548717151No Hit
ATCCAGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT5900.10083005350828772No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5860.10014645992518069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)