FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424469
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20190.47565311012111605No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17220.40568333612113017No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA16710.39366832442416294No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG16380.38589390509083116No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15140.35668093547467544No Hit
GGGCTATACCGCCCGCTTCTCCGCCTCTTGTACCGTCCATCCACGGGGCG14890.350791223858515No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14750.3474929853534652No Hit
CCCAAAGTCAGGGCCCTATACTTTCCTGCTGGTGGCTCCAGTTCAGGAAC13460.31710207341407737No Hit
GTATAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCTG13020.306736180969635No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT11390.26833526123226903No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10940.25773378032318023No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10870.2560846610706553No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCTG9600.22616492606056035No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9410.22168874523227847No Hit
ACGTACACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC9350.22027521444439993No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9180.21627021054541085No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9100.2143855028282395No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8800.20731784888884702No Hit
ATATGTTCCTGTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAA8730.2056687296363221No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8590.20237049113127226No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7740.1823454716363268No Hit
CTGTTTACGTAGAAAGGCCTTGTAAATTGGCGAGAAAGTAAAAGCCTGTT7720.18187429470703395No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA7690.1811675293130947No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7650.18022517545450903No Hit
GGTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA7570.17834046773733772No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC7540.17763370234339845No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7530.17739811387875204No Hit
GTGGTAATGTGCCCCAACTCCCAATTACATATCCCATGAAGTTAAGGGAA7520.17716252541410563No Hit
CAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA7310.17221516765653086No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7290.17174399072723803No Hit
CATTAGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCA7240.17056604840400594No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7110.16750339836360253No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC7060.16632545604037044No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7020.16538310218178479No Hit
CATTACCACAGGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAA7000.16491192525249193No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCAC6910.16279162907067418No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCAAGGAACCTCTATG6850.16137809828279567No Hit
CGTACACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCT6790.1599645674949172No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGTAAATATTTAGTAACATT6700.15784427131309944No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6690.157608682848453No Hit
GTAAATTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGTATACATGCATATA6690.157608682848453No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6580.15501720973734243No Hit
GTACAAGAGGCGGAGAAGCGGGCGGTATAGCCCCAAGCGGCCCCGGGAGG6540.15407485587875674No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6520.15360367894946392No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6500.1531325020201711No Hit
TCTATATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATT6330.149127498121182No Hit
CTCTTGTACCGTCCATCCACGGGGCGCACCTCTCTTTACGCGGACTCCCC6270.1477139673333035No Hit
ACGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC6270.1477139673333035No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCATTAAACAAACAGTCTTTGA6110.14394455189896083No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6080.14323778650502156No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC6050.14253102111108232No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6040.1422954326464359No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6030.1420598441817895No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGTAAATA5940.13993954799997174No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5940.13993954799997174No Hit
ATATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5900.13899719414138606No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTAAGGCA5690.1340498363838113No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5660.13334307098987205No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG5660.13334307098987205No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5630.1326363055959328No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC5580.13145836327270072No Hit
ATCCTAATGTTACTAAATATTTACCCTTAGATAAAGGGATCAAACCGTAT5560.1309871863434079No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5510.1298092440201758No Hit
CGACCGAATTGTCAGTCCCGATGAGTTTTGCCCCAGACCTGCTGCGAGCA5350.12603982858583312No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5270.12415512086866179No Hit
GCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCCTATGGCCAAG5170.12179923622219761No Hit
CCCTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAA5020.11826540925250137No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4990.11755864385856211No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4950.11661628999997645No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC4940.11638070153533002No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGGAAAGAAGGAGTTA4910.11567393614139077No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4800.11308246303028018No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4790.11284687456563378No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4770.11237569763634092No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4730.11143334377775527No Hit
ATATTTACCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAG4700.11072657838381603No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4670.11001981298987677No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4640.1093130475959375No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4640.1093130475959375No Hit
CTGTGTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4590.10813510527270542No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4580.10789951680805901No Hit
CCCAGAGTCAGGGCCCTATACTTTCCTGCTGGTGGCTCCAGTTCAGGAAC4580.10789951680805901No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT4570.1076639283434126No Hit
CTGTGTACGTAGAAAGGCCTTGTAAATTGGCGAGAAAGTAAAAGCCTGTT4550.10719275141411976No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4530.10672157448482693No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC4520.1064859860201805No Hit
CCTACTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4480.10554363216159483No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4470.10530804369694843No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4450.10483686676765558No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA4450.10483686676765558No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4440.10460127830300918No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4440.10460127830300918No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTTCA4430.10436568983836275No Hit
ATGTAATTGGGAGTTGGGGCACATTACCACAGGAACATATTGTACAAAAA4380.10318774751513066No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4380.10318774751513066No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4370.10295215905048426No Hit
GGGCTGTACCGCCCGCTTCTCCGCCTCTTGTACCGTCCATCCACGGGGCG4340.102245393656545No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGGGGAGTTCCACTTCA4310.10153862826260573No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4300.10130303979795933No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4280.10083186286866651No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA4280.10083186286866651No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4270.10059627440402008No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA4250.10012509747472724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)