FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491820
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAAAATCTCCTCGACACCGCCTCTGCTTTGTATCGGGAGGCCTTAGA30340.6168923589931276No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA20560.4180391200032533No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC16250.3304054328819487No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15830.3218657232320768No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15640.3180025212476109No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT14120.28709690537188404No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA13320.2708307917530804No Hit
TAATTAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCATCC11670.23728193241429793No Hit
ATTCGAGATCTCCTCGACACCGCCTCTGCTTTGTATCGGGAGGCCTTAGA10980.22325240941807978No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10570.21491602618844294No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT10130.20596966369810094No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9880.2008865031922248No Hit
GATTCATTAACTCACCCCAACACAGAATAGCTTGCCTGAGTGCCGTATGG9810.1994632182505795No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA9730.1978366068886991No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9550.1941767313244683No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9290.18889024439835714No Hit
GATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGAGTAACTCC8790.17872392338660487No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8500.17282745719978854No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTAGGGGAGGAGGTTA8460.17201415151884836No Hit
CCTTTGCCCTAACCTCCTCCCCTAACTCCTCCCACTCGTTAAACACACAG8460.17201415151884836No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8390.17059086657720304No Hit
CCTTTAACCTAACCTCCTCCCCTAACTCCTCCCACTCGTTAAACACACAG8120.1651010532308568No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8070.1640844211296816No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7990.16245780976780125No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG7990.16245780976780125No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7910.1608311984059209No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7850.1596112398845106No Hit
CACCTATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT7760.1577813021023952No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7750.15757797568216014No Hit
CCCCTAACTCCTCCCACTCGTTAAACACACAGTCTTTGAAGTATGCCTCA7620.15493473221910456No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7460.1516815094953438No Hit
CCTGTAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7430.15107153023463868No Hit
GTTTACAGGAAGTTTCCTAAAACATATTTTGATTTTTTGTACAATATGTT6880.1398885771217112No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6840.139075271440771No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC6820.1386686186003009No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6540.13297547883371966No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6460.1313488674718393No Hit
GGAACATATTGTACAAAAAATCAAAATATGTTTTAGGAAACTTCCTGTAA6440.1309422146313692No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6400.13012890895042903No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC6380.12972225610995894No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6210.12626570696596315No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6130.12463909560408279No Hit
CCCACAAAATGAAGCGCTACGTGTGGACTCTCTTTTATATAAGATCCCCG5960.12118254646008703No Hit
GCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCTTGAGG5920.12036924077914685No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5890.1197592615184417No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5860.11914928225773658No Hit
GCTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5820.1183359765767964No Hit
GTGCTGGTGAACACACCTATTTATGCCTACAGCCTCCTAGTACAAAGACC5810.11813265015656134No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5800.11792932373632631No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5780.11752267089585622No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5760.11711601805538613No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5650.11487942743280061No Hit
GGCCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG5550.11284616323045016No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTGTTTAACGAGT5550.11284616323045016No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5550.11284616323045016No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5530.11243951038998007No Hit
TGGAAACCACCGTGAACGCCCACGGGAATCTGCCCAAGGTCTTGCATAAG5510.11203285754950998No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5430.11040624618762962No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5430.11040624618762962No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5420.11020291976739457No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5390.10959294050668944No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5380.1093896140864544No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5340.10857630840551422No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5320.10816965556504413No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5260.10694969704363386No Hit
GTTGTGAGCAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5260.10694969704363386No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5220.10613639136269368No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5110.10389980074010817No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5100.10369647431987314No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5030.1022731893782278No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5020.10206986295799277No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4920.10003659875564232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)