FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491820
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAAAATCTCCTCGACACCGCCTCTGCTTTGTATCGGGAGGCCTTAGA26160.5319019153348786No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA20130.40929608393314626No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15770.32064576471066647No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14920.3033630189906876No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12720.2586312065389777No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT12500.25415802529380666No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA12200.24805823268675534No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10710.21776259607173354No Hit
ATTCGAGATCTCCTCGACACCGCCTCTGCTTTGTATCGGGAGGCCTTAGA10330.21003619210280183No Hit
TAATTAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCATCC10320.20983286568256682No Hit
GATTCATTAACTCACCCCAACACAGAATAGCTTGCCTGAGTGCCGTATGG9740.19803993330893416No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT9450.19214346712211786No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA9310.18929689723882723No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9210.18726363303647678No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9070.18441706315318612No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT8950.18197714611036558No Hit
CCTTTAACCTAACCTCCTCCCCTAACTCCTCCCACTCGTTAAACACACAG8880.18055386116872027No Hit
CCTTTGCCCTAACCTCCTCCCCTAACTCCTCCCACTCGTTAAACACACAG8870.18035053474848523No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8760.1781139441258997No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTAGGGGAGGAGGTTA8640.17567402708307917No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8520.17323411004025863No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8280.16835427595461755No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8070.1640844211296816No Hit
GATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGAGTAACTCC7910.1608311984059209No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG7400.15046155097393354No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7340.14924159245252328No Hit
CCCCTAACTCCTCCCACTCGTTAAACACACAGTCTTTGAAGTATGCCTCA7240.14720832825017283No Hit
CACCTATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT7060.14354845268594202No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7040.14314179984547193No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6990.14212516774429668No Hit
GTTTACAGGAAGTTTCCTAAAACATATTTTGATTTTTTGTACAATATGTT6780.13785531291936073No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6730.1368386808181855No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC6540.13297547883371966No Hit
GCTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6540.13297547883371966No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6500.13216217315277948No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6470.13155219389207434No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6470.13155219389207434No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6410.13033223537066405No Hit
CCTGTAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6390.12992558253019396No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6330.1287056240087837No Hit
GGAACATATTGTACAAAAAATCAAAATATGTTTTAGGAAACTTCCTGTAA5930.12057256719938188No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5860.11914928225773658No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC5660.11508275385303567No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5630.11447277459233052No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5620.11426944817209549No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5560.11304948965068522No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5550.11284616323045016No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5540.11264283681021513No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5490.11162620470903989No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5430.11040624618762962No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5400.10979626692692449No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5390.10959294050668944No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5360.1089829612459843No Hit
GTGCTGGTGAACACACCTATTTATGCCTACAGCCTCCTAGTACAAAGACC5330.10837298198527916No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5160.10491643284128341No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5160.10491643284128341No Hit
GTTGTGAGCAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5150.10471310642104835No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5130.10430645358057826No Hit
GGCCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG5090.10349314789963808No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5040.10247651579846286No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4990.10145988369728762No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4980.10125655727705259No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4940.1004432515961124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)