FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565226
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA30440.5385456436894268No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC26230.4640621627455213No Hit
GAGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT17040.3014723314214137No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16480.2915647900131983No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG15890.2811264874581141No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG15700.2777650001946124No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT15140.26785745878639694No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14480.2561807135552859No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14170.25069618170430946No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT13070.23123493965245762No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA12980.2296426562118515No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT12980.2296426562118515No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA12570.22238892053797948No Hit
CCCTTTACTCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTGT12430.21991203518592564No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG12350.21849667212760915No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG12340.21831975174531956No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTACAA12310.2177889905984509No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG11970.2117736976006058No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT11890.2103583345422893No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC11630.20575840460276065No Hit
ATATTAACGTTGACATAGCTTACTACTAATTCCCTGGATGCCGGGTCTTC10980.19425857975393912No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC10610.18771252560922533No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAAATTAGACAACTATT10480.18541256063946104No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10290.18205107337595935No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTA10000.17692038228956206No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC9830.17391273579063948No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC9740.17232045235003343No Hit
CATGCATACAAAGGCATTAATGCTGGGTAACCACATTGAGTAAAGGGAGC9690.1714358504385856No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9670.1710820096740065No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA9640.17055124852713782No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT9490.1678974427927944No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9470.16754360202821528No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG9340.16524363705845097No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCT9230.16329751285326577No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC9170.1622359905595284No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC8950.15834374214915803No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC8840.15639761794397286No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACCTTT8820.15604377717939374No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA8700.15392073259191896No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA8440.14932080265239037No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT8390.14843620074094258No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8320.14719775806491564No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGACCTTTAACCTAATCTCCTCC8290.14666699691804694No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC8170.14454395233057218No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT8050.14242090774309746No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8020.14189014659622876No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7850.1388825000973062No Hit
ACACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG7840.13870557971501665No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7790.13782097780356883No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC7770.13746713703898972No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTAGG7760.13729021665670016No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTGTAGGAAGTTTCCTAAAAC7720.1365825351275419No Hit
GCATTAATGCCTTTGTATGCATGTGTACAAGCTAAGCAGGCTTTCACTTT7670.1356979332160941No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7630.13499025168693585No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7620.13481333130464626No Hit
CACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG7580.13410564977548803No Hit
CCTTTACCCCGTCGCGCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7560.1337518090109089No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGCCAGGTCTTGCCT7540.13339796824632977No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC7440.13162876442343416No Hit
GTACACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGAGTAAAG7380.13056724212969678No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7360.13021340136511766No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA7340.12985956060053855No Hit
CAATAGGTCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGT7320.12950571983595943No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA7070.1250827102787204No Hit
ATGTTCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCA7030.1243750287495621No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6990.12366734722040387No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6980.1234904268381143No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6940.12278274530895605No Hit
GTCCTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6900.12207506377979781No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6810.12048278033919176No Hit
GTATGCATGTGTACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6760.11959817842774395No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6750.11942125804545439No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6690.11835973575171702No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6670.1180058949871379No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6650.11765205422255877No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6630.11729821345797964No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG6580.11641361154653183No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6410.11340596504760926No Hit
GTCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATA6330.11199060198929277No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6250.11057523893097627No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6230.11022139816639716No Hit
GGTCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT6210.10986755740181803No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6180.10933679625494935No Hit
CCACAATAGTTGTCTAATTTTTAGGCCCATATTAACGTTGACATAGCTTA6150.10880603510808066No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA6150.10880603510808066No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTCGCGCGGCAACGGTCAGGTC6110.10809835357892242No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6100.10792143319663286No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA6090.10774451281434329No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6080.10756759243205373No Hit
TCCCAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT6070.10739067204976417No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6060.10721375166747461No Hit
CATTAATGCTGGGTAACCACATTGAGTAAAGGGAGCGGCAAAACCCAAAA6050.10703683128518504No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6010.1063291497560268No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG6010.1063291497560268No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5980.10579838860915812No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT5950.10526762746228943No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5950.10526762746228943No Hit
AGCTTGTACACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGAG5910.10455994593313116No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTG5870.10385226440397291No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACAC5800.10261382172794599No Hit
GATCCATACTGCGGAACTCCTAGCCGCCTGTTTTGCTCGCAGCTGGTCTG5770.1020830605810773No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5770.1020830605810773No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5760.10190614019878774No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5750.10172921981649817No Hit
ACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG5750.10172921981649817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)