FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565226
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA25890.4580468697476761No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC22430.3968324174754877No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG19190.33951021361366956No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16300.28838022313198614No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA15800.27953420401750806No Hit
GAGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT15660.2770573186654542No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG14240.25193462438033637No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13890.24574241100020167No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT13430.23760407341488185No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA13380.23671947150343403No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG13100.2317657007993263No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA13010.23017341735872024No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT12590.22274276130255863No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11820.20911989186626231No Hit
CCCTTTACTCAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTGT11650.20611224536733977No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA11240.19885850969346774No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTACAA11040.19532010204767652No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT10820.19142785363730613No Hit
ATATTAACGTTGACATAGCTTACTACTAATTCCCTGGATGCCGGGTCTTC10790.19089709249043746No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC10550.18665100331548798No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA10520.18612024216861928No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG10460.18505871987488193No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC10440.18470487911030278No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10370.18346643643427585No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT10320.18258183452282803No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG9280.16418211476471356No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG9250.16365135361784489No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9200.1627667517063971No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAAATTAGACAACTATT9190.1625898313241075No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC9130.16152830903037016No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8810.1558668567971042No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT8700.15392073259191896No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8670.1533899714450503No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8570.15162076762215468No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCT8480.15002848418154863No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGCCAGGTCTTGCCT8370.14808235997636343No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8340.14755159882949476No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8140.1440131911837035No Hit
ATGTTCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCA8010.1417132262139392No Hit
CCTTTACCCCGTCGCGCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7930.1402978631556227No Hit
CATGCATACAAAGGCATTAATGCTGGGTAACCACATTGAGTAAAGGGAGC7480.13233644595259242No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC7480.13233644595259242No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC7480.13233644595259242No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC7470.13215952557030286No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTA7390.13074416251198637No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGACCTTTAACCTAATCTCCTCC7350.1300364809828281No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7320.12950571983595943No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7280.12879803830680117No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC7270.1286211179245116No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT7180.12702883448390556No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA7120.12596731219016818No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7080.12525963066100995No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA7060.12490578989643082No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7040.12455194913185168No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTGTAGGAAGTTTCCTAAAAC6960.12313658607353518No Hit
GTCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATA6940.12278274530895605No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA6930.1226058249266665No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6920.12242890454437694No Hit
GCATTAATGCCTTTGTATGCATGTGTACAAGCTAAGCAGGCTTTCACTTT6890.12189814339750825No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6850.12119046186835No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC6820.12065970072148131No Hit
GTACACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGAGTAAAG6720.1188904968985857No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTCGCGCGGCAACGGTCAGGTC6710.11871357651629613No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6670.1180058949871379No Hit
CTGCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGT6640.11747513384026921No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6550.11588285039966315No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC6550.11588285039966315No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6500.11499824848821534No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6490.11482132810592575No Hit
CAATAGGTCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGT6480.1146444077236362No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6460.11429056695905708No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6440.11393672619447795No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT6410.11340596504760926No Hit
GTATGCATGTGTACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6380.11287520390074059No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6360.11252136313616146No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC6310.11163676122471365No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6270.1109290796955554No Hit
GTCCTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6270.1109290796955554No Hit
GGTCTATTTGTAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT6100.10792143319663286No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6060.10721375166747461No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6000.10615222937373724No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA6000.10615222937373724No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC5950.10526762746228943No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTG5810.10279074211023555No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACCTTT5780.10225998096336686No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5750.10172921981649817No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5750.10172921981649817No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5740.10155229943420861No Hit
ACACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG5720.1011984586696295No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT5710.10102153828733992No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT5710.10102153828733992No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA5680.10049077714047125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)