FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483426
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCTACAATTCTTTGACATACT36550.7560619412278198No Hit
CCCTTTACACAGTGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT21820.4513617389217791No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG20680.42778005320359264No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG20180.417437208590353No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT17970.37172183539983367No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT15200.3144224762424859No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT15060.3115264797507788No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15050.31131962285851406No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC14930.30883734015133646No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG13970.28897907849391635No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13790.28525565443315004No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12650.2616739687149636No Hit
ATCCTAACCGTACCAAGTATTTGCCCTTAGATAAAGGCATCAAACCTTAT11760.243263705303397No Hit
ACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATG11740.2428499915188674No Hit
ATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC11250.23271400379789253No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC10810.22361230053824163No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10570.21864773512388658No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG10280.2126488852482076No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA10050.20789117672611734No Hit
CATCTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10010.20706374915705816No Hit
CTCCAAAAGACACTAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9930.2054088940189398No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTAGGTCT9920.20520203712667504No Hit
CCCTTAACTTCATGGGCTACGTAATTGGAAGTTGGGGTACCTTACCACAG9800.20271975441949752No Hit
AAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCT9430.19506604940570016No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACACTGTGTAAAG9160.18948091331455072No Hit
GTAACCACACTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCTACAATTC9050.187205487499638No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTGTAA8670.17934492559357587No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTGTC8670.17934492559357587No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8660.17913806870131108No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8470.17520778774828No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG8420.17417350328695602No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8410.17396664639469123No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8250.17065693611845453No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT8220.17003636544166015No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8130.16817465341127702No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA8040.16631294138089386No Hit
GTACATTCTTAGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7870.16279637421239238No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATACTTGGTA7820.16176208975106843No Hit
GTTCTATCCTAACCGTACCAAGTATTTGCCCTTAGATAAAGGCATCAAAC7780.16093466218200925No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACGTAGCCCATGAA7680.1588660932593613No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA7670.15865923636709653No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG7530.1557632398753894No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7480.15472895541406545No Hit
CCTTACCACAGGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAA7460.15431524162953586No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7450.15410838473727106No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATACTTGGTACGGTT7330.15162610203009355No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA7240.1497643899997104No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7140.14769582107706247No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6930.1433518263395018No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6930.1433518263395018No Hit
ATGTGGTATTGGGGGCTAAGAATGTACAACATCTTGAATCCCTTTTTACC6910.14293811255497224No Hit
GTGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGC6820.14107640052458909No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6600.13652554889476362No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6590.13631869200249883No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6590.13631869200249883No Hit
AACCTTACCAAGTATTTGCCCTTAGATAAAGGCATCAAACCTTATTATCC6570.13590497821796926No Hit
GTTTAAATGTATACCCAAAGATAAAAGAAAATTGGTAATAGCGGTAAAAA6490.1342501230798509No Hit
ACACTAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATG6490.1342501230798509No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAC6450.13342269551079172No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA6400.13238841104946777No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6380.13197469726493818No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTAGGTCT6360.13156098348040857No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA6190.1280444163119071No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6070.1255621336047296No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5920.12245928022075767No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5910.1222524233284929No Hit
TAACCACACTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCTACAATTCT5810.12018385440584495No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT5760.11914956994452097No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC5720.11832214237546182No Hit
GCTTTATTCTTCTACGGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT5680.11749471480640263No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5550.11480557520696033No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5550.11480557520696033No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5520.11418500453016593No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5370.11108215114619405No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5350.11066843736166444No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5270.1090135822235461No Hit
CCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCG5250.1085998684390165No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA5230.10818615465448694No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5150.1065312995163686No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGT5100.10549701505504462No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAA5060.10466958748598545No Hit
GTGGTAAGGTACCCCAACTTCCAATTACGTAGCCCATGAAGTTAAGGGAG5060.10466958748598545No Hit
ACGTAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACAAAAA5050.10446273059372066No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5050.10446273059372066No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAAC5020.10384215991692627No Hit
GCACAGGACCCTGCAAGACCTGCACGAGTCCTGCTCCAAGAAACTCTACG4980.10301473234786711No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA4980.10301473234786711No Hit
GTACAAAGATCATTAACCGAGTCTCCTCCCCCAACTCCTCCCAGTCCTTA4930.10198044788654313No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA4930.10198044788654313No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCTAAGAATGTACAACATCTTG4910.10156673410201353No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4910.10156673410201353No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4840.10011873585615999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)