FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483426
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCTACAATTCTTTGACATACT48941.0123576307438988No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG26410.5463090524713193No Hit
CCCTTTACACAGTGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT19620.4058532226235246No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG18900.39095952638045944No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT17960.37151497850756887No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC15830.3274544604551679No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14930.30883734015133646No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14200.2937367870160066No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT13980.28918593538618115No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG12980.2685002461597018No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC12110.25050369653266474No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT11560.23912656745810112No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11390.23561000028959966No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACACTGTGTAAAG10850.2244397281073008No Hit
CCCTTAACTTCATGGGCTACGTAATTGGAAGTTGGGGTACCTTACCACAG10560.2184408782316218No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG10490.21699287998576824No Hit
ATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC10210.21120088700235404No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA9850.20375403888082147No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9750.20168546995817352No Hit
CTCCAAAAGACACTAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9730.20127175617364393No Hit
ACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATG9430.19506604940570016No Hit
GTAACCACACTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCTACAATTC9350.1934111942675818No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9300.19237690980625782No Hit
ATCCTAACCGTACCAAGTATTTGCCCTTAGATAAAGGCATCAAACCTTAT9210.1905151977758747No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACGTAGCCCATGAA8960.18534377546925485No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATACTTGGTA8690.17975863937810543No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8670.17934492559357587No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8600.17789692734772228No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG8580.17748321356319272No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA8530.17644892910186874No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA8470.17520778774828No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTAGGTCT8350.17272550504110246No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8290.1714843636875137No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8260.1708637930107193No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG8250.17065693611845453No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATACTTGGTACGGTT8060.16672665516542345No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTGTC7630.15783180879803735No Hit
GTGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGC7620.15762495190577255No Hit
CATCTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7570.15659066744444858No Hit
AAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCT7300.15100553135329917No Hit
GTTTAAATGTATACCCAAAGATAAAAGAAAATTGGTAATAGCGGTAAAAA7250.1499712468919752No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTGTAA7220.1493506762151808No Hit
GTACATTCTTAGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7200.14893696243065122No Hit
GTTCTATCCTAACCGTACCAAGTATTTGCCCTTAGATAAAGGCATCAAAC7180.14852324864612163No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAC7120.14728210729253288No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA7010.14500668147762014No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6870.14211068498591303No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6860.14190382809364824No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6850.14169697120138344No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6790.1404558298477947No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6750.13962840227873555No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6650.1375598333560876No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC6530.13507755064891006No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6510.13466383686438047No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6260.12949241455776064No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA6260.12949241455776064No Hit
ATGTGGTATTGGGGGCTAAGAATGTACAACATCTTGAATCCCTTTTTACC6190.1280444163119071No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6180.1278375594196423No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAA6170.1276307025273775No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6100.12618270428152395No Hit
CCTTACCACAGGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAA5960.12328670778981685No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5870.1214249957594337No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5790.11977014062131536No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC5680.11749471480640263No Hit
GTGGTAAGGTACCCCAACTTCCAATTACGTAGCCCATGAAGTTAAGGGAG5640.11666728723734347No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5640.11666728723734347No Hit
ACACTAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATG5580.11542614588375469No Hit
GCTTTATTCTTCTACGGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT5550.11480557520696033No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5500.11377129074563634No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5400.11170272182298843No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5260.1088067253312813No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5260.1088067253312813No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5230.10818615465448694No Hit
TAACCACACTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCTACAATTCT5200.10756558397769256No Hit
AACCTTACCAAGTATTTGCCCTTAGATAAAGGCATCAAACCTTATTATCC5160.10673815640863336No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5130.106117585731839No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTAGGTCT5100.10549701505504462No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA5100.10549701505504462No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4970.10280787545560231No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4950.10239416167107272No Hit
GAATATGGTGACCCACAAAATGAGGCGCTGCGAGTAGTTTCTCTCTTATA4920.10177359099427834No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4910.10156673410201353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)