FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354030
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG16060.45363387283563544No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT13400.3784989972601192No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT13050.36861282942123547No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT12560.35477219444679825No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12000.33895432590458435No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT11700.33048046775696976No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11110.31381521339999435No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9410.2657966838968449No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9260.2615597548230376No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9110.2573228257492303No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA8710.24602434821907748No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8630.2437646527130469No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7460.21071660593734992No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7430.2098692201225885No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7390.2087393723695732No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7190.2030901336044968No Hit
TAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATCATC7100.2005479761602124No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7040.1988532045306895No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6910.19518119933338984No Hit
GGGTTATTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACAT6720.18981442250656724No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6440.18190548823546027No Hit
TTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAACCCGCACAAGGA6400.180775640482445No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6390.18049317854419117No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6370.17992825466768353No Hit
GCTGTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6350.1793633307911759No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6340.17908086885292207No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6220.17569132559387624No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6130.17314916814959183No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6000.16947716295229218No Hit
TGATCAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG5960.1683473151992769No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT5920.16721746744626162No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5910.1669350055080078No Hit
GGCTCTGAACGCTGGGTCCAACTGATGATCGGGGAAGAATCCCAGGGGAT5810.1641103861254696No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA5790.16354546224896194No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5700.16100330480467756No Hit
CATTTAAACCCTGACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT5620.158743609298647No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5530.15620145185436263No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT5450.15394175634833207No Hit
GACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCATTAAACACAC5440.15365929441007825No Hit
TTGTCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC5380.15196452278055533No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG5370.15168206084230149No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG5350.15111713696579385No Hit
GTTCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAAGTTA5330.1505522130892862No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAA5300.14970482727452475No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA5230.147727593706748No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5160.14575036013897127No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5150.14546789820071745No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5150.14546789820071745No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTGTTGTGGTTTCACATTTCCT5100.14405558850944833No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5080.14349066463294072No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA4970.1403835833121487No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4940.13953619749738722No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4790.13529926842357992No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4790.13529926842357992No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTG4690.13247464904104173No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4640.13106233934977263No Hit
CACAAGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCT4640.13106233934977263No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAA4630.1307798774115188No Hit
GGGATATTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACAT4580.1293675677202497No Hit
GTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4580.1293675677202497No Hit
GATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATT4450.12569556252295003No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4350.12287094314041183No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4330.12230601926390419No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4320.12202355732565037No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4310.12174109538739654No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAAATGATTAACT4260.12032878569612744No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4240.1197638618196198No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4230.119481399881366No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4230.119481399881366No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGT4160.11750416631358925No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4120.11637431856057397No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4100.11580939468406631No Hit
GCTTTGCCCCAGACCTGCTGCGAGCAAAGCAAGCGGCTAGGAGTTCCGCA4050.11439708499279722No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGCTT4010.11326723723978194No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4010.11326723723978194No Hit
GATCATTAACCTAACCTCCTCCCCCAACTCCTCCCACTCATTAAACACAC3970.11213738948676667No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3920.11072507979549755No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG3920.11072507979549755No Hit
CTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT3870.10931277010422845No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3840.10846538428946699No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA3830.10818292235121318No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC3830.10818292235121318No Hit
GAATATCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3800.10733553653645171No Hit
GCTTAGCCCCAGACCTGCTGCGAGCAAAGCAAGCGGCTAGGAGTTCCGCA3800.10733553653645171No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3770.10648815072169025No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3720.10507584103042115No Hit
GAATACAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAACATTGAGAT3680.10394599327740586No Hit
CCCTAACAAAACAAAAAGATGGGGATATTCCCTTAACTTCATGGGATATG3650.10309860746264442No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3650.10309860746264442No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG3650.10309860746264442No Hit
GGAATATCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAG3640.10281614552439058No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA3590.10140383583312149No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3590.10140383583312149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)