FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301779
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13670.4529804923470487No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12680.42017502874620166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11760.389689143379758No Hit
GTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCAT10550.3495935767565006No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9740.3227527429012622No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9580.3174508497940546No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8160.2703965484675872No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA8070.26741423359478295No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7910.26211234048757537No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7800.2584672889763701No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7590.25150855427316016No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7010.2322891917595326No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6980.2312950868019312No Hit
GAATGCTGGGTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGA6510.2157207757995089No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6400.2120757242883037No Hit
GATAAGATAGGGGCATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCA6290.20843067277709848No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6200.2054483579042942No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6050.2004778331162871No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5750.19053678354027287No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5700.1888799419442705No Hit
CCCTTACAGTAAATGAAAACCGGAGACTAAAATTAATTATGCCTGCTAGG5430.17993299732585766No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5400.17893889236825625No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5380.17827615572985528No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5360.17761341909145434No Hit
CTCTTGGACTCTTGCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACG5240.17363699926104864No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5190.1719801576650463No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAAGCC4980.1650214229618363No Hit
GTACAAAGATCATTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAG4930.16336458136583396No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4880.16170773976983158No Hit
CCCATTATTCAGAGCCAACTCAGAAAATCCAGATTGGGACCTCAACCCGC4700.15574311002422303No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4640.15375490010902018No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4550.1507725852362159No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4540.15044121691701542No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4520.14977848027861448No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4470.1481216386826121No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG4470.1481216386826121No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4460.14779027036341164No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAACCCCC4440.1471275337250107No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4350.14414521885220644No Hit
CAATCACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4300.14248837725620406No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4220.13983743070260024No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4220.13983743070260024No Hit
TATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGCAGGT4190.13884332574499883No Hit
AGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGT4180.13851195742579836No Hit
GCATAAGAGGACTCTTGGACTCTTGCATAAGAGGACTCTTGGACTTTCAG4170.13818058910659786No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4170.13818058910659786No Hit
CACAATGCCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGGAG4110.13619237919139504No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4090.13552964255299407No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4010.13287869599939028No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA3970.13155322272258838No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTAGGTTAATGATCTTTGTAC3940.13055911776498696No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3930.1302277494457865No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3910.12956501280738553No Hit
GTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTA3840.1272454345729822No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC3830.12691406625378174No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3810.1262513296153808No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG3810.1262513296153808No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACGGGAACCTGCCC3810.1262513296153808No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3670.12161217314657415No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG3660.12128080482737366No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC3630.12028669986977225No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3620.11995533155057178No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3600.11929259491217084No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC3600.11929259491217084No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3480.11531617508176513No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACCGGAGACTA3450.11432207012416372No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3430.11365933348576275No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3420.11332796516656228No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3420.11332796516656228No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC3380.11200249188976039No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA3330.11034565029375801No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT3310.10968291365535707No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC3300.1093515453361566No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3290.10902017701695613No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3290.10902017701695613No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG3280.10868880869775566No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT3280.10868880869775566No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC3270.10835744037855517No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3260.10802607205935469No Hit
GACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTCTGAAG3220.1067005987825528No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3160.10471238886734995No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3130.10371828390974852No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3120.10338691559054805No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG3110.10305554727134758No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3090.10239281063294661No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC3090.10239281063294661No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC3090.10239281063294661No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3020.10007323239854332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)