FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301779
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14210.47087438158387424No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13570.449666809155044No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11750.3893577750605576No Hit
GTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGCAT10480.3472739985220973No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10290.34097800045728827No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8970.29723738232282565No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8190.27139065342518864No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7680.2544908691459644No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7470.24753213444275446No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7440.24653802948515305No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6730.22301087882191936No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6720.2226795105027189No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6520.21605214411870938No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6220.20611109454269516No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6200.2054483579042942No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5500.182252575560261No Hit
GATAAGATAGGGGCATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCA5340.1769506824530534No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5250.1739683675802491No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5210.17264289430344723No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5140.1703233160690439No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA4920.16303321304663346No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4870.1613763714506311No Hit
CCCTTACAGTAAATGAAAACCGGAGACTAAAATTAATTATGCCTGCTAGG4860.1610450031314306No Hit
CTCTTGGACTCTTGCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACG4830.16005089817382917No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4810.15938816153542823No Hit
GAATGCTGGGTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGA4710.1560744783434235No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAACCCCC4670.1547490050666216No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4600.15242942683221827No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4570.15143532187461686No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4520.14977848027861448No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4460.14779027036341164No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4430.14679616540581022No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4400.1458020604482088No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG4350.14414521885220644No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4300.14248837725620406No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTAGGTTAATGATCTTTGTAC4280.1418256406178031No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4250.14083153566020165No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4200.1391746940641993No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4150.13751785246819692No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4080.1351982742337936No Hit
GTACAAAGATCATTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAG4070.13486690591459313No Hit
AGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGT4020.13321006431859075No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3910.12956501280738553No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG3840.1272454345729822No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3840.1272454345729822No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3790.12558859297697986No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3780.1252572246577794No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAAGCC3760.1245944880193784No Hit
GTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTA3720.12326901474257652No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3720.12326901474257652No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA3700.12260627810417558No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3680.12194354146577462No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC3640.12061806818897272No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC3620.11995533155057178No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT3570.1182984899545694No Hit
TATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGCAGGT3480.11531617508176513No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3400.11266522852816134No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC3370.11167112357055992No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG3330.11034565029375801No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3280.10868880869775566No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG3280.10868880869775566No Hit
GCATAAGAGGACTCTTGGACTCTTGCATAAGAGGACTCTTGGACTTTCAG3270.10835744037855517No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC3190.10570649382495138No Hit
CACAATGCCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGGAG3190.10570649382495138No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA3170.10504375718655042No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC3150.10438102054814948No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC3140.104049652228949No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACCG3130.10371828390974852No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC3110.10305554727134758No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC3100.1027241789521471No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA3090.10239281063294661No Hit
CAATCACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG3060.1013987056753452No Hit
GCCAATTAGGATTAAAGCAAGGTACCGTAGAAGAATAAAGCCCCGTAAAG3050.10106733735614473No Hit
CCCATTATTCAGAGCCAACTCAGAAAATCCAGATTGGGACCTCAACCCGC3050.10106733735614473No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT3040.10073596903694426No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3020.10007323239854332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)