FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529098
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15540.2937074039213907No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13190.24929219161667593No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13170.24891418980982732No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10080.1905129106517129No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9930.18767789710034813No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9710.18351987722501314No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT9300.17577084018461608No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9030.1706678157921595No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8970.16953381037161358No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8900.16821080404764335No Hit
CTCCAAAAGACACCAAATATTCAAGGACAGTTTCTCTCCCAAAAGTAAGA8790.16613179410997586No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8710.1646197868825813No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8470.16008376520039766No Hit
GGGTTATTCCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACAT8100.1530907317736979No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7990.1510117218360304No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7710.14571969654014946No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7650.14458569111960354No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7590.14345168569905764No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7480.14137267576139015No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7460.1409946739545415No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7460.1409946739545415No Hit
CGTTAATATGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCT7070.1336236387209931No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7030.13286763510729582No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6810.1287096152319608No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6630.1253075989703231No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6560.12398459264635285No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6420.12133857999841238No Hit
ATATAAAAGAGAGTCCACACGTAGTGCCTCATTTTGCGGGTCACCATATT6380.12058257638471512No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6360.12020457457786649No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6350.12001557367444216No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6240.11793656373677466No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTGCGGGAGAGCACGACAGAAT6160.1164245565093801No Hit
GCCTTAGAGTGTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6090.11510155018540989No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5790.10943152308268034No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5710.1079195158552858No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5710.1079195158552858No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAACTAA5580.10546250411076964No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5580.10546250411076964No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5570.10527350320734533No Hit
CATGTATACAAGCAAAACAGGCTTTTAGTTTCTCGCCAACTTACAAGGCC5500.1039504968833751No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTAGTTT5480.10357249507652647No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5410.10224948875255625No Hit
GAATAACCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGA5380.10168248604228328No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5330.1007374815251617No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTCATCT5310.10035947971831305No Hit
GTATCGGGAGGCCTTAGAGTGTCCGGAACATTGTTCACCTCACCATACGG5300.10017047881488872No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)