FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494870
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGAGT28660.5791419968880716No Hit
TCCAAAGAGTGTGTAAATAATGTCGAGTCTGAAAGTAATGATTAACTACA21730.4391052195526097No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT19160.3871723887081456No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA16960.34271626891911006No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15520.3136177177844686No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG14060.28411502010629053No Hit
TTTCCTGACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATT13740.27764867540970356No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12790.25845171459171096No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12750.2576434215046376No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12700.2566330551457959No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11930.24107341321963344No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10560.21338937498737043No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG10490.21197486208499203No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10180.2057105906601734No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10160.20530644411663668No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9980.2016691252248065No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGA9860.19924424596358642No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9040.18267423767858226No Hit
GTGTAAATAATGTCGAGTCTGAAAGTAATGATTAACTACATACTCTGGAT9030.1824721644068139No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8980.1814617980479722No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8850.17883484551498371No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTTCAGACTCGACATTATT8540.1725705740901651No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA8110.16388142340412634No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8100.163679350132358No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA7970.16105239759936954No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7890.15943581142522278No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7530.15216117364156242No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7500.1515549538262574No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7490.15135288055448906No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7480.1511508072827207No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7400.14953422110857395No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT7400.14953422110857395No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7280.14710934184735386No Hit
GTGTGTAAATAATGTCGAGTCTGAAAGTAATGATTAACTACATACTCTGG7270.1469072685755855No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7180.14508860912967042No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA6940.14023885060723018No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6840.13821811788954674No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6770.13680360498716834No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6650.13437872572594822No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6550.13235799300826478No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA6530.1319538464647281No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6470.13074140683411806No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT6470.13074140683411806No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC6370.12872067411643462No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6370.12872067411643462No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTTTTGGGGGC6270.12669994139875118No Hit
ATCCAGAGTATGTAGTTAATCATTACTTTCAGACTCGACATTATTTACAC6100.12326469577868937No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6090.12306262250692102No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6060.12245640269161599No Hit
GTCATGAAAAGAAGGAGTTTGCCATTTAGGATTAAAGCAAGGTACCGTAG6000.12124396306100592No Hit
GCTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC6000.12124396306100592No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5930.11982945015862753No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5900.11922323034332248No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5860.1184149372562491No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5700.11518176490795562No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT5650.11417139854911391No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5620.11356517873380888No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5610.11336310546204054No Hit
CAGTTATATGGATGATATGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5540.11194859255966214No Hit
GTTATATGGATGATATGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5400.10911956675490532No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5290.10689676076545356No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5280.10669468749368521No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCATTAC5280.10669468749368521No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5260.10629054095014853No Hit
CTTCCAAAGAGTGTGTAAATAATGTCGAGTCTGAAAGTAATGATTAACTA5220.10548224786307515No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC5210.1052801745913068No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5200.10507810131953846No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC5150.10406773496069675No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT5070.10245114878655No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5070.10245114878655No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT5060.10224907551478167No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC5050.10204700224301332No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGTTACGT5030.10164285569947663No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4980.10063248934063491No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4960.10022834279709823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)