FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494870
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGAGT28030.566411380766666No Hit
TCCAAAGAGTGTGTAAATAATGTCGAGTCTGAAAGTAATGATTAACTACA19930.402732030634308No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT18420.37221896659728815No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15370.3105866187079435No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG14630.2956331965970861No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA14450.2919958777052559No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12700.2566330551457959No Hit
TTTCCTGACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATT12550.2536019560692707No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12180.24612524501384203No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11820.23885060723018167No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11660.23561743488188816No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10510.2123790086285287No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG10390.20995412936730856No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGA10210.2063168104754784No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTTCAGACTCGACATTATT10050.2030836381271849No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9980.2016691252248065No Hit
GTGTAAATAATGTCGAGTCTGAAAGTAATGATTAACTACATACTCTGGAT9960.20126497868126983No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9810.1982338796047447No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9710.19621314688706126No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9680.1956069270717562No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT9490.1917675349081577No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8490.17156020773132338No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8290.16751874229595654No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA8200.16570008285004142No Hit
GTGTGTAAATAATGTCGAGTCTGAAAGTAATGATTAACTACATACTCTGG8050.16266898377351627No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7810.15781922525107603No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7700.15559641926162426No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7690.15539434598985594No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT7610.15377775981570918No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7540.15236324691333078No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7380.1491300745650373No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA7250.14650312203204882No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7200.1454927556732071No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6900.1394305575201568No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6880.13902641097662014No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6770.13680360498716834No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6740.1361973851718633No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6680.13498494554125326No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6640.13417665245417987No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6570.13276213955180147No Hit
GTCATGAAAAGAAGGAGTTTGCCATTTAGGATTAAAGCAAGGTACCGTAG6470.13074140683411806No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTTTTGGGGGC6290.12710408794228786No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6230.12589164831167782No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6130.12387091559399438No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6060.12245640269161599No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA6000.12124396306100592No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5900.11922323034332248No Hit
ATCCAGAGTATGTAGTTAATCATTACTTTCAGACTCGACATTATTTACAC5850.11821286398448078No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT5840.11801079071271244No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA5830.11780871744094408No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5820.11760664416917575No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5800.11720249762563906No Hit
GCTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC5630.11376725200557723No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5540.11194859255966214No Hit
GTTATATGGATGATATGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5500.11114029947258877No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5440.1099278598419787No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5390.10891749348313699No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5340.10790712712429526No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5240.10588639440661184No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA5220.10548224786307515No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5170.10447188150423345No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT5140.1038656616889284No Hit
GTATGTAGTTAATCATTACTTTCAGACTCGACATTATTTACACACTCTTT5120.10346151514539172No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC5120.10346151514539172No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5090.1028552953300867No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5080.10265322205831834No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5060.10224907551478167No Hit
CAGTTATATGGATGATATGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5000.10103663588417161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)