FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511036
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40920.8007263676140233No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT27650.5410577728379214No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT24990.4890066453243998No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT23130.45260999225103515No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA22320.4367598368803763No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA20200.39527547961396065No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT19500.3815778144788234No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC18640.36474925445565476No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA18590.363770849803145No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT18260.3573133790965803No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA16870.33011372975680775No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA16820.32913532510429794No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA16070.3144592553166509No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG14720.2880423296988862No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA13140.25712474267957636No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT13060.2555592952355607No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG12950.25340680500003915No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT12090.23657824497687052No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT11940.23364303101934109No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC11710.22914236961779602No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA11360.2222935370502274No Hit
GTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT11300.22111945146721562No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG11230.2197496849537019No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG11220.21955400402319994No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG11060.21642310913516857No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG10960.21446629983014895No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC10720.2097699574981019No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT10670.20879155284559212No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10230.20018159190350582No Hit
CTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTG10120.19802910166798424No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10090.19744205887647837No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC9870.19313707840543523No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT9770.19118026910041563No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9620.18824505514288623No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC9580.18746233142087837No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA9570.1872666504903764No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA9450.18491847932435287No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT9330.18257030815832936No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC9320.18237462722782738No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC9310.18217894629732542No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA9090.17787396582628232No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA9090.17787396582628232No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8900.17415602814674502No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8850.17317762349423524No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8730.1708294523282117No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG8510.1665244718571686No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8430.16495902441315288No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA8310.16261085324712937No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT8250.1614367676641176No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAAC8120.1588929155675921No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG8020.15693610626257248No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7980.15615338254056466No Hit
GGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGAT7940.15537065881855683No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7680.15028295462550584No Hit
CTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACT7660.14989159276450192No Hit
GTTCAGGAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA7510.14695637880697252No Hit
CTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTAT7390.14460820764094898No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT7310.1430427601969333No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT7290.14265139833592935No Hit
GGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAA7270.14226003647492544No Hit
GTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAA7220.14128163182241563No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7210.1410859508919137No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA7180.1404989081004078No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG7020.13736801321237643No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG7010.13717233228187448No Hit
ATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG7000.13697665135137252No Hit
TTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA7000.13697665135137252No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6950.1359982466988627No Hit
CTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTG6910.13521552297685485No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA6820.1334543946023372No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT6790.1328673518108313No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA6690.13091054250581172No Hit
AGTCTAGACTCTGTGGTATTATGAGGATTTTTGTCAACAAGAAAAACCCC6670.1305191806448078No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC6650.1301278187838039No Hit
CCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAAC6640.1299321378533019No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA6600.12914941413129408No Hit
CTCCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGT6500.12719260482627448No Hit
GTATTATGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGC6350.12425739086874507No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC6260.12249626249422742No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6140.12014809132820388No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA6100.11936536760619604No Hit
GGATATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACA6070.11877832481469017No Hit
CATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAA6060.1185826438841882No Hit
ATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACA6030.11799560109268233No Hit
GAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCAC6020.11779992016218035No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6020.11779992016218035No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5980.11701719644017251No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5940.11623447271816467No Hit
TACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG5920.11584311085716076No Hit
CATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGG5870.11486470620465095No Hit
CGGTTGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC5860.11466902527414898No Hit
ATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATACCCAAAGACAAA5670.11095108759461172No Hit
CCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAA5650.11055972573360781No Hit
CAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAA5470.1070374689845725No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5470.1070374689845725No Hit
TCAACAACCAGCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGG5390.10547202154055682No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5380.10527634061005488No Hit
GTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAAC5350.104689297818549No Hit
CTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATA5270.10312385037453331No Hit
CACTTGGCACAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT5220.1021454457220235No Hit
GGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAAT5180.10136272200001566No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG5130.10038431734750584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)