FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450772
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29920.6637501885653945No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT12130.26909391000328325No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10010.2220634822038636No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTTGTAACATT8410.18656881971373554No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8200.18191014526190624No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCAACCAATCGGCAGT7550.16749043862529173No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7350.1630536058140257No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6250.1386510253520627No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT6140.13621076730586637No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6030.13377050925967007No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5790.12844630988615086No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5780.1282244682455876No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5410.12001632754474545No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5180.11491396981178956No Hit
GTTTACAGGGAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5090.11291739504671984No Hit
GTATTAACGTTGACATAACTGACTACTAATTCCCTGGATGCTGGATCTTC4970.11025529535996025No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG4790.10626214582982084No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4720.10470925434587774No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4700.10426557106475112No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4660.10337820450249795No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4590.10182531301855482No Hit
CATGTATACAAGCGAAACGGGCTTTTACTTTCTCGCCAACTTACAAGGCC4580.10160347137799153No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG4540.10071610481573834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)