FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450772
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29750.6599788806758183No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT11770.26110761094300444No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10210.22650031501512963No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTTGTAACATT9670.21452086642471138No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8180.18146646198077965No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8100.17969172885627324No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7260.161057031048956No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6730.14929942409910107No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCAACCAATCGGCAGT6630.1470810076934681No Hit
GTTTACAGGGAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6040.13399235090023337No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5770.12800262660502426No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT5660.12556236855882796No Hit
GTTTTATCCCAATGTTACAAAATATTTGCCCTTAGATAAAGGGATCAAAC5620.12467500199657476No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5530.12267842723150506No Hit
GTATTAACGTTGACATAACTGACTACTAATTCCCTGGATGCTGGATCTTC5460.12112553574756196No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5210.11557949473347945No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC4920.10914608715714375No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4880.10825872059489054No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG4840.10737135403263735No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA4800.10648398747038414No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG4510.10005057989404843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)