FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503306
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27690.5501623266958868No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15250.30299658656960177No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA13280.2638553881733975No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12410.24656968126745954No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGTTT11940.2372314258125276No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA11640.2312708372242731No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10370.2060376788673292No Hit
GTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT10200.20266001200065167No Hit
ATCCTAACCTTACTAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10140.2014678942830008No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9900.19669942341239724No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9770.19411650169082031No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG9290.18457955994961317No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCCATGTCAACGACCGACCT9130.18140057936921078No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8690.17265838277310425No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG8540.169678088478977No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8390.16669779418484976No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8360.16610173532602432No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTAGTAAGGTT7460.14821996956126093No Hit
TTCGTAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGA7460.14821996956126093No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG7200.14305412611810708No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6970.13848434153377864No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6910.13729222381612777No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6810.13530536095337628No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT6800.13510667466710113No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6650.13212638037297392No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6430.12775528207492062No Hit
ACCTCCAATCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCG6430.12775528207492062No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCTGAGAGTCCAA6420.12755659578864548No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6330.12576841921216914No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA6330.12576841921216914No Hit
CATTCCTTGATTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACT6300.1251723603533437No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6250.12417892892196795No Hit
CTCTTACAGTGAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6250.12417892892196795No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA6230.12378155634941765No Hit
GTTTTATCCTAACCTTACTAAATATTTGCCCTTGGATAAAGGCATTAAAC6220.1235828700631425No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6060.12040388948274011No Hit
CAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTAT6030.11980783062391466No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6020.11960914433763953No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6000.11921177176508924No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5970.1186157129062638No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT5950.11821834033371348No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5760.11444330089448566No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT5660.11245643803173419No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5640.11205906545918387No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5620.11166169288663358No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT5590.11106563402780813No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5310.10550241801210397No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5310.10550241801210397No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5270.10470767286700337No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5220.10371424143562763No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5220.10371424143562763No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5160.10252212371797674No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5140.10212475114542645No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGGTTAAAG5120.10172737857287616No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5060.10053526085522525No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5050.10033657456895011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)