FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503306
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27510.5465859735429341No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16270.32326258776966693No Hit
GTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14520.2884924876715159No Hit
TCCACAGAGTGTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA12780.2539210738596401No Hit
CTTATATAGGATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGTTT12740.25312632871453944No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11260.22372075834581748No Hit
GTGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT11200.22252864062816657No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10290.20444818857712804No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9990.19848759998887358No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCCATGTCAACGACCGACCT9440.1875598542437404No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8840.17563867706723146No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8750.17385050049075512No Hit
ATCCTAACCTTACTAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8750.17385050049075512No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8660.17206232391427878No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8190.16272406845934678No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG7900.1569621661573675No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG7840.1557700484397166No Hit
TTCGTAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGA7700.15298844043186452No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG7620.15139895014166332No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTAGTAAGGTT7600.15100157756911303No Hit
GTATACACGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7530.14961077356518698No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7500.14901471470636155No Hit
CTCTTATATAGGATGCCAGCCTTCCACAGAGTGTGTAAATAATGCCTAGT7360.14623310669850947No Hit
ACCTCCAATCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCG7320.14543836155340886No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7100.1410672632553556No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6990.13888171410632896No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6890.13689485124357748No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6830.13570273352592657No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6450.12815265464747094No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCTGAGAGTCCAA6310.12537104663961884No Hit
CTCTTACAGTGAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6180.1227881249180419No Hit
CTTTATATGCGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACT6180.1227881249180419No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6170.12258943863176677No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCGTGTATA6150.12219206605921645No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA5900.11722490890233775No Hit
CATTTACAGGAGGACATTATTAATAGGTGTCAACAATATGTGGGCCCTCT5790.11503935975331112No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5780.11484067346703596No Hit
GTTTTATCCTAACCTTACTAAATATTTGCCCTTGGATAAAGGCATTAAAC5770.11464198718076082No Hit
CAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTAT5660.11245643803173419No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTT5610.11146300660035842No Hit
CATTCCTTGATTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACT5600.11126432031408329No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5580.11086694774153298No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5550.11027088888270753No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGGTTAAAG5490.10907877116505664No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5490.10907877116505664No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5460.10848271230623122No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5460.10848271230623122No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5440.1080853397336809No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5420.1076879671611306No Hit
CGTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5350.10629716315720455No Hit
ATATAAGAGGACTCTTGGACTCTCAGCCATGTCAACGACCGACCTTGAGG5330.10589979058465426No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5290.10510504543955368No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATTAATAATGTCCTCCTGT5270.10470767286700337No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5230.10391292772190279No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5090.1011313197140507No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5050.10033657456895011No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5040.10013788828267495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)