FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504998
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCAATAATGTCCTCCCGTAAATGAATGTTAGGAAAGGAGGGAGTTTG50691.0037663515499071No Hit
AATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGA31260.6190123525241684No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA30980.6134677761100044No Hit
ATCTCAATGTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG27780.5501011885195586No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC22000.43564528968431554No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21490.4255462397870883No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20540.4067342840961746No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG19750.39109065778478336No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19620.3885163901639214No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16980.3362389554018036No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCGACTCCTCCCAGTC16420.32514980257347553No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA15990.3166349173660094No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15400.30495170277902095No Hit
GGACCTTGACACGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTT15380.3045556616065806No Hit
TTATTGATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA15220.3013873322270583No Hit
CTATTAATAATGTCCTCCCGTAAATGAATGTTAGGAAAGGAGGGAGTTTG15160.3001992087097375No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14630.28970411764006987No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA14400.28514964415700655No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT13890.2750505942597793No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA13790.2730703883975778No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC13530.2679218531558541No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG12670.25089208274092173No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG12300.24356532105077644No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG12120.24000095049881384No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11940.2364365799468513No Hit
CTGTTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTG11810.23386231232598942No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11740.23247616822244843No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11490.22752565356694482No Hit
CATGTATACAATCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCA11040.21861472718703837No Hit
AACCCTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCAT10950.21683254191105708No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG10810.21406025370397505No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10750.21287213018665418No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGT10550.20891171846225132No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAATGCCTTGTAAGTTGGCGA9940.1968324627028226No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC9680.19168392746109886No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9540.18891163925401686No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9510.1883175774953564No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9240.18297102166741255No Hit
TCTCAATGTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGC9210.1823769599087521No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9140.18099081580521112No Hit
CATTAAGGTTCCCGAGATTGAGATCGTCTGCGACGCGGCGATTGAGACCT8900.17623832173592766No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGCTGGGGTACTTTACCACAG8750.17326801294262553No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA8730.17287197177018523No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8670.17168384825286437No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8630.1708917659079838No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8630.1708917659079838No Hit
ACCTTAATGTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG8620.17069374532176365No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC8540.1691095806320025No Hit
ATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATGAA8300.16435708656271905No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8130.16099073659697663No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7990.15821844838989463No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7960.15762438663123418No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT7940.15722834545879388No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACCTCCCCC7710.1526738719757306No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7640.1512877278721896No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATAAG7570.1499015837686486No Hit
TTACATAACCAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7520.14891148083754788No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7460.147723357320227No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7450.14752533673400686No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG7390.146337213216686No Hit
GGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7360.14574315145802558No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG7340.1453471102855853No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7330.14514908969936513No Hit
GTCGATGAGATCTCGAATGGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA7310.14475304852692486No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGCGAGGCCTTAGAGTCTCCGGA7230.1431688838371637No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7080.14019857504386157No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6970.13802034859543996No Hit
TATTGATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA6930.1372282662505594No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTGTACCTGTC6730.13326785452615653No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA6700.1326737927674961No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6610.13089160749151482No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATG6610.13089160749151482No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6530.12930744280175369No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6410.12693119576711195No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6250.12376286638758965No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACGGG6110.12099057818050765No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCCGT6010.11901037231830622No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5990.11861433114586592No Hit
CAGGACGACAGCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCT5970.11821828997342565No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5850.11584204293878392No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTCA5830.11544600176634362No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCT5830.11544600176634362No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5800.11485194000768321No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT5790.11465391942146305No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG5750.11386183707658248No Hit
AAGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA5740.11366381649036235No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5730.11346579590414219No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5680.11247569297304148No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5590.11069350769706018No Hit
GCTCTGTATCGCGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5520.10930736359351918No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGTAGATGAGAAGGCACAG5490.10871330183485875No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5430.10752517831753788No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5400.10693111655887745No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC5360.10613903421399688No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5330.10554497245533645No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC5290.10475289011045588No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5240.10376278717935516No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5230.10356476659313502No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5180.10257466366203431No Hit
CAACTACCAGCACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGA5160.10217862248959401No Hit
GTGCACACGGTCCGGTAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5160.10217862248959401No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5160.10217862248959401No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5140.10178258131715373No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5130.10158456073093358No Hit
ATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGAT5110.1011885195584933No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5110.1011885195584933No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5060.10019841662739258No Hit
GGCCTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG5060.10019841662739258No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5050.10000039604117245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)