FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504998
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCAATAATGTCCTCCCGTAAATGAATGTTAGGAAAGGAGGGAGTTTG51751.0247565336892424No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA30600.6059429938336389No Hit
AATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGA27630.5471308797262564No Hit
ATCTCAATGTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG25840.5116851947928507No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG24040.47604148927322487No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20310.40217981061311137No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC20160.3992095018198092No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG20110.39821939888870844No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19730.390694616612343No Hit
TTATTGATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA17130.3392092641951057No Hit
CTATTAATAATGTCCTCCCGTAAATGAATGTTAGGAAAGGAGGGAGTTTG16600.3287141731254381No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16210.3209913702628525No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA15770.3122784644691662No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15600.30891211450342376No Hit
GGACCTTGACACGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTT14470.28653578826054754No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13820.27366445015623825No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT13490.2671297708109735No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA13390.26514956494877207No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG13360.2645555031901116No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12960.2566346797413059No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA12780.25307030918934337No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC12780.25307030918934337No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCGACTCCTCCCAGTC12750.25247624743068287No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12260.24277323870589587No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11830.2342583534984297No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11700.23168408587756784No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC11390.22554544770474338No Hit
CTGTTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAACATACCTTG10420.20633745084138946No Hit
CATGTATACAATCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCA10310.2041592243929679No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG10250.20297110087564704No Hit
ACACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG10080.1996047509099046No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9810.1942581950819607No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9670.1914859068748787No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9610.19029778335755784No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG9600.19009976277133772No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAATGCCTTGTAAGTTGGCGA9550.18910965984023698No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGCTGGGGTACTTTACCACAG9460.18732747456425572No Hit
TCTCAATGTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGC9240.18297102166741255No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9220.18257498049497226No Hit
AACCCTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCAT8880.17584228056348739No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8760.17346603352884565No Hit
CATTAAGGTTCCCGAGATTGAGATCGTCTGCGACGCGGCGATTGAGACCT8610.1704957247355435No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACCTCCCCC8520.16871353945956222No Hit
ATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATGAA8410.16653531301114063No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8290.16415906597649893No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8290.16415906597649893No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGT8280.16396104539027875No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA8220.16277292187295792No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGCGAGGCCTTAGAGTCTCCGGA8130.16099073659697663No Hit
GGATAATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7970.15782240721745433No Hit
GGATAATAGGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7770.15386199549305146No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7640.1512877278721896No Hit
ACCTTAATGTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7640.1512877278721896No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG7500.1485154396651076No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7490.14831741907888743No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7470.14792137790644716No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT7450.14752533673400686No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7410.1467332543891263No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7090.1403965956300817No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7060.13980253387142128No Hit
TATTGATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA6990.13841638976788026No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATAAG6980.13821836918166014No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6980.13821836918166014No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG6910.13683222507811912No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6700.1326737927674961No Hit
GTCGATGAGATCTCGAATGGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA6700.1326737927674961No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6690.13247577218127596No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6640.13148566925017524No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACGGG6630.13128764866395512No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6470.12811931928443282No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6300.12475296931869037No Hit
GCTCTGTATCGCGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6260.1239608869738098No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTGTACCTGTC6220.12316880462892922No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6170.1221787016978285No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCT6090.12059453700806735No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA6090.12059453700806735No Hit
GGCCTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG6060.12000047524940692No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6040.11960443407696665No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTCA6030.1194064134907465No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6000.11881235173208607No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC5970.11821828997342565No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5910.11703016645610478No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATG5830.11544600176634362No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5820.11524798118012348No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5790.11465391942146305No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5760.11405985766280262No Hit
TTACATAACCAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5760.11405985766280262No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG5660.11207965180060118No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCCTAT5620.11128756945572062No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCCGT5600.11089152828328032No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5530.10950538417973932No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5490.10871330183485875No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5490.10871330183485875No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5370.10633705480021702No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTACCGGACCGTGTG5260.10415882835179545No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5260.10415882835179545No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5220.10336674600691488No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5170.10237664307581415No Hit
GTCTATTGACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA5100.10099049897227316No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5100.10099049897227316No Hit
ATATGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGAT5050.10000039604117245No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5050.10000039604117245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)