FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653736
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40790.6239521764137206No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG35760.5470098021219575No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT35280.5396673886706561No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT27930.42723668269760273No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG26300.4023030703525582No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC23600.3610019946889876No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTTTGACATACT21700.33193827477758603No Hit
CCTTTAAATGTATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG19020.29094313300781965No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT19010.29079016606091757No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17930.2742697357954893No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAACGGAGATTAAAATTAAT17000.26004380973359276No Hit
CCTGTAAATGTATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG16440.25147766070707445No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT16160.24719458619381524No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA15930.24367634641506664No Hit
CATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT15590.2384754702203948No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCA15300.23403942876023348No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA14100.2156833951319799No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC14100.2156833951319799No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC14090.21553042818507778No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14020.214459659556763No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT13810.2112473536718186No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13200.20191636991078965No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13140.20099856822937698No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA12560.1921264853090544No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA12320.1884552785834037No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11950.1827955015480255No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG11810.1806539642913959No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11110.169946278008248No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAACGGAGATTAAAATTAAT10900.1667339721233036No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10880.16642803822949936No Hit
TGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGG10730.16413353402596767No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC10320.15786188920298103No Hit
ATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT10040.15357881468972184No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9730.1488368393357563No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9600.14684826902602885No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG9490.1451656326101056No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9350.14302409535347602No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG9180.1404236572561401No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT9120.13950585557472742No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9110.1393528886278253No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC9050.13843508694641263No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8990.13751728526499996No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC8810.13476388022076188No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATTTGTGGGCCCTCT8730.13354014464554498No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8700.13308124380483866No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA8640.13216344212342596No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8580.13124564044201328No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCATTAAC8400.12849223539777524No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8380.12818630150397103No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT8350.12772740066326468No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCT8260.12635069814114566No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8060.1232913592031034No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC8050.12313839225620128No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG8040.12298542530929918No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCAACTTACAAGGCC8010.12252652446859283No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG7800.11931421858364846No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7650.11701971438011674No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7620.11656081353941039No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7600.11625487964560617No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT7580.11594894575180194No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7330.11212477207924912No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7250.11090103650403221No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7230.11059510261022798No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7230.11059510261022798No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG7190.10998323482261953No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTGTACCTGTC7090.1084535653535984No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7090.1084535653535984No Hit
TTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA7090.1084535653535984No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7010.1072298297783815No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6840.10462939168104557No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG6770.10355862305273078No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6750.10325268915892655No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATACATTTACAGG6680.10218192053061173No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6620.10126411884919906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)