FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653736
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG41000.627164482298665No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40420.6182923993783423No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT31610.48352851915758044No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT28220.43167272415776403No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG25620.3919013179632145No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21830.3339268450873136No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTTTGACATACT18740.2866600584945605No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT18630.28497742207863724No Hit
CCTTTAAATGTATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG17790.2721281985388597No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCA17530.26815105791940475No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17020.260349743627397No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT16750.2562196360610399No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT15910.24337041252126243No Hit
CCTGTAAATGTATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG15490.23694580075137361No Hit
CATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT14700.22486141194610668No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAACGGAGATTAAAATTAAT14230.21767196544170736No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA14130.21614229597268622No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT14060.21507152734437143No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA13960.2135418578753503No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13880.2123181223001334No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA13860.21201218840632918No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13550.20727021305236368No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC12620.1930442869904671No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA12540.1918205514152502No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12280.18784341079579525No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC12080.18478407185775297No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA11870.18157176597280858No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC11220.17162891442417122No Hit
TGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGG10580.16183902982243598No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10410.15923859172510005No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10400.15908562477819793No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10350.15832079004368735No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG10110.15464958331803663No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC10100.15449661637113454No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA10010.15311991384901552No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG9910.15159024437999438No Hit
ATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT9460.14470673176939927No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9410.1439418970348887No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8880.1358346488490767No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8780.13430497938005553No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC8770.13415201243315344No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8700.13308124380483866No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAACGGAGATTAAAATTAAT8690.13292827685793654No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8500.13002190486679638No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA8480.12971597097299215No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8210.1255858634066351No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8210.1255858634066351No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCATTAAC8040.12298542530929918No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7800.11931421858364846No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA7730.11824344995533365No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATTTGTGGGCCCTCT7670.11732564827392096No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7660.11717268132701884No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7650.11701971438011674No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7460.11411334238897658No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7440.11380740849517236No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7360.11258367291995545No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7320.111971805132347No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7240.1107480695571301No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7170.10967730092881531No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG7150.10937136703501107No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCAACTTACAAGGCC7130.10906543314120686No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6880.10524125946865402No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6800.10401752389343712No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA6770.10355862305273078No Hit
TTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA6760.10340565610582866No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6720.10279378831822018No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6720.10279378831822018No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6670.10202895358370963No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG6590.10080521800849272No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6580.1006522510615906No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6570.10049928411468849No Hit
GATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT6540.10004038327398215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)