FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385237
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20650.5360336623948375No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT18530.4810026035920745No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT15660.40650300983550386No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG14910.3870344748816962No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14410.37405545157915776No Hit
CCTTTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG13890.36055726734451776No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13780.35770188221795934No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11280.29280676570526715No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11080.2876151563842518No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT10670.27697235727617026No Hit
GTGTAAAAGGGGCGGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10260.26632955816808873No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10250.266069977702038No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT10020.26009962698287026No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9960.2585421441865657No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9680.25127389113714416No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG9300.24140983342721495No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9070.23543948270804724No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC9040.23466074130989492No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8540.22168171800735653No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8290.2151922063560873No Hit
ATCTCAGTCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG7700.19987695885909196No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7530.19546409093622888No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7310.18975332068311196No Hit
TTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT7240.18793625742075656No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7220.18741709648865504No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7080.18378296996394428No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7010.18196590670158888No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6720.17443807318611662No Hit
ATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT6720.17443807318611662No Hit
CCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA6690.1736593317879643No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6680.17339975132191354No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6570.17054436619535507No Hit
GACTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA6460.16768898106879662No Hit
GGAACATATTGTCCTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA6460.16768898106879662No Hit
ACAATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6370.1653527568743397No Hit
TTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCT6310.16379527407803507No Hit
GTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCT6230.16171863034962894No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6210.16119946941752739No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6180.1604207280193751No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6060.15730576242676586No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5530.14354799772607513No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5420.14069261259951665No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5360.13913512980321205No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5340.13861596887111052No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5280.1370584860748059No Hit
GTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGATCTCC5220.1355010032785013No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5220.1355010032785013No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA5140.13342435955009516No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5130.13316477908404437No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5120.1329051986179936No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5090.1321264572198413No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5080.13186687675379052No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA5060.131347715821689No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5030.13056897442353668No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4980.12927107209328284No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4950.12849233069513055No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4950.12849233069513055No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4830.12537736510252132No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4780.12407946277226746No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4750.12330072137411514No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGGACAATA4740.12304114090806438No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGGACAAT4730.12278156044201363No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGG4720.12252197997596286No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4660.12096449717965824No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4630.12018575578150593No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4490.11655162925679517No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4480.11629204879074441No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAGGACAATATGTT4470.11603246832469363No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4350.1129175027320844No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4290.1113600199357798No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4220.10954295667342441No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG4170.10824505434317057No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4130.10720673247896749No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4120.10694715201291671No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4080.10590883014871363No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT4010.10409176688635827No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4010.10409176688635827No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT3970.10305344502215519No Hit
CATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTGCA3950.10253428409005365No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3940.10227470362400289No Hit
CATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT3900.1012363817597998No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT3900.1012363817597998No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3860.10019805989559673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)